Rabbit Proteasome 19S, Subunit S5A Polyclonal Antibody | anti-RPN10 antibody

Proteasome 19S, Subunit S5A (PSMD4)

Cat. Num. AAA618137

• Gene Names: RPN10; MCB1; SUN1
• Reactivity: Human, Monkey, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Synonyms: Proteasome 19S; Subunit S5A; Polyclonal Antibody; Subunit S5A (PSMD4); Anti -Proteasome 19S; anti-RPN10 antibody

• Host: Rabbit
• Reactivity: Human, Monkey, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total human S5a/PSMD4 protein, 49kD. Species Crossreactivity: Monkey, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by Protein A and immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

• Applicable Applications for anti-RPN10 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000
• Immunogen: Synthetic peptide corresponding to residues surrounding Gly344 of human S5a/PSMD4 protein (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of extracts from various cell types using MBS618137.)

Related Product Information for anti-RPN10 antibody

S5a(PSMD4) is a subunit of the 19S regulatory proteasome complex functioning in ubiquitinylated-protein targeting and degradation. S5a contains two polyubiquitin binding motifs (UIM) which bind multiubiquitin chains by hydrophobic interaction. In addition to ubiquitin, the UIM of S5a shows high affinity to a ubiquitin-like domain present in many proteins. S5a binds to these types of protein directly and mediates their targeting to the proteasome for degradation.

Product Categories/Family for anti-RPN10 antibody

Antibodies; Abs to Proteasomes

NCBI and Uniprot Product Information

• NCBI GI #: 398365027
• NCBI GeneID: 856607
• NCBI Accession #: NP_012070.3
• NCBI GenBank Nucleotide #: NM_001179331.3
• UniProt Accession #: P38886; D3DLE9
• Molecular Weight: 49kD
• NCBI Official Full Name: proteasome regulatory particle base subunit RPN10
• NCBI Official Symbol: RPN10
• NCBI Official Synonym Symbols: MCB1; SUN1
• NCBI Protein Information: proteasome regulatory particle base subunit RPN10
• UniProt Protein Name: 26S proteasome regulatory subunit RPN10
• Protein Family: 26S proteasome regulatory
• UniProt Gene Name: RPN10
• UniProt Synonym Gene Names: MCB1; SUN1
• UniProt Entry Name: RPN10_YEAST

Uniprot Description

Function: Multiubiquitin binding protein.

Subunit structure: The 26S proteasome is composed of a core protease, known as the 20S proteasome, capped at one or both ends by the 19S regulatory complex (RC). The RC is composed of at least 18 different subunits in two subcomplexes, the base and the lid, which form the portions proximal and distal to the 20S proteolytic core, respectively

By similarity.

Miscellaneous: Present with 17200 molecules/cell in log phase SD medium.

Sequence similarities: Belongs to the proteasome subunit S5A family.Contains 1 UIM (ubiquitin-interacting motif) repeat.Contains 1 VWFA domain.