Rabbit anti-Mouse, Rat Natural Resistance Associated Macrophage Protein with IRE Polyclonal Antibody | anti-Slc11a1 antibody

Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE) (formerly M1252-06)

Cat. Num. AAA617851

• Gene Names: Slc11a1; Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
• Reactivity: Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Natural Resistance Associated Macrophage Protein with IRE; Polyclonal Antibody; Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE) (formerly M1252-06); Anti -Natural Resistance Associated Macrophage Protein with IRE (Nramp2 with IRE) (formerly M1252-06); anti-Slc11a1 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat NRAMP2 with IRE
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.1% sodium azide.

• Applicable Applications for anti-Slc11a1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1-10ug/ml (ECL).; ELISA: 0.5-1ug/ml. Coat ELISA plates at 1ug/ml of Control Peptide.
• Immunogen: Synthetic peptide corresponding to 17aa located near the C-terminal, cytoplasmic domain of rat NRAMP2 conjugated with KLH. This sequence is 100% homologous with mouse Nramp2 with IRE and is absent in NRAMP2 without IRE. No significant sequence homology exists with NRAMP1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Slc11a1 antibody

Iron is an essential element for a variety physiological process. A variety of proteins are involved in the transport of iron. Most dietary iron exists in the form of ferric iron complexes which must be reduced to yield ferrous ion before it can be taken up by protein transporters such as DCT1 (divalent cation transporter)/NRAMP2/DMT1. These transporters are especially active in small intestine where most dietary iron absorption is conducted. Ferrous Fe (II) is very unstable at physiological pH and quickly oxidized to ferric Fe (III). Highly specialized transmembrane electron transport system, maintained by ferric reductases, is required for the availability of intracellular ferrous ion. A second member of NRAMP family, termed NRAMP2/DMT/DCT1 (Divalent Metal ion Transporter 1 or Divalent Cation Transporter 1), has been identified (human, rat and mouse 568aa, ~65% identity with NRAMP1). NRAMP2 expression is more ubiquitous and has been detected in most tissues. NRAMP2 expression is more pronounced in brain, thymus, proximal intestine, kidney and bone marrow. It is dramatically up regulated by iron starvation in the intestine. NRAMP2 gene produces two alternatively spliced transcripts generated by alternative use of two 3' exons encoding distinct C-termini of the protein as well as distinct 3' untranslated regions (UTRs). One Nramp2 mRNA contains an iron-responsive element (IRE) in its 3'UTR. The IRE is an RNA secondary structure present in the 5'- or the 3'-UTR of animal mRNAs encoding proteins involved in iron metabolism. The second Nramp2 splice isoform (without-IRE, isoform II) encodes a protein in which the C-terminal 18aa of the IRE form (with IRE, isoform I) is replaced by a novel 25aa segment and codes for a distinct 3' UTR lacking the IRE. The two isoforms are differentially localized and regulated in GI tract and kidney. It has been demonstrated that the Nramp2 gene is mutated (Gly185 to Arg at TM4) in both the mk and Belgrade (b) animal models exhibiting a severe microcytic hypochromic anemia marked by a defect in iron absorption by intestinal cells and in erythroid iron use.

Product Categories/Family for anti-Slc11a1 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 505156
• NCBI GeneID: 18173
• UniProt Accession #: P41251; Q3TB84
• Molecular Weight: 59,741 Da
• NCBI Official Full Name: natural resistance associated macrophage protein
• NCBI Official Synonym Full Names: solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1
• NCBI Official Symbol: Slc11a1
• NCBI Official Synonym Symbols: Bcg; Ity; Lsh; ity; Nramp; Nramp1; MGC129156; MGC129157
• NCBI Protein Information: natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh
• UniProt Protein Name: Natural resistance-associated macrophage protein 1
• Protein Family: Natural resistance-associated macrophage protein
• UniProt Gene Name: Slc11a1
• UniProt Synonym Gene Names: Bcg; Ity; Lsh; Nramp1
• UniProt Entry Name: NRAM1_MOUSE

Uniprot Description

SLC11A1: Divalent transition metal (iron and manganese) transporter involved in iron metabolism and host resistance to certain pathogens. Macrophage-specific membrane transport function. Controls natural resistance to infection with intracellular parasites. Pathogen resistance involves sequestration of Fe(2+) and Mn(2+), cofactors of both prokaryotic and eukaryotic catalases and superoxide dismutases, not only to protect the macrophage against its own generation of reactive oxygen species, but to deny the cations to the pathogen for synthesis of its protective enzymes. Belongs to the NRAMP family.

Protein type: Transporter, SLC family; Cell surface; Membrane protein, multi-pass; Membrane protein, integral; Transporter; Vesicle

Cellular Component: phagocytic vesicle membrane; cell surface; membrane; lysosome; late endosome; integral to membrane; plasma membrane; endosome membrane; vacuole

Molecular Function: transition metal ion transmembrane transporter activity; protein homodimerization activity; transporter activity; manganese ion transmembrane transporter activity; metal ion:hydrogen antiporter activity

Biological Process: respiratory burst; positive regulation of T-helper 1 type immune response; wound healing; cellular iron ion homeostasis; positive regulation of dendritic cell antigen processing and presentation; manganese ion transport; response to lipopolysaccharide; defense response to Gram-negative bacterium; antigen processing and presentation of peptide antigen; vacuolar acidification; nitrite transport; T cell cytokine production; T cell proliferation during immune response; transport; positive regulation of phagocytosis; interleukin-3 production; inflammatory response; positive regulation of cytokine production; mRNA stabilization; MAPKKK cascade; L-arginine import; defense response to protozoan; iron ion transport; interleukin-2 production; phagocytosis; iron ion homeostasis; macrophage activation; cellular cadmium ion homeostasis; MHC class II biosynthetic process; positive regulation of interferon-gamma production; response to bacterium; protein import into nucleus, translocation; defense response to bacterium; negative regulation of cytokine production; activation of protein kinase activity; ion transport; positive regulation of transcription from RNA polymerase II promoter