Western Blot (WB)
(Western Blot of DAMI cell lysate using MBS617444 (0.5ug/ml).)
Rabbit anti-Human Thrombopoietin Receptor Antibody | anti-MPL antibody
Thrombopoietin Receptor (c-Mpl) (TPO)
IHC: Stains megakaryocytes in bone marrow biopsy sections.
IP: 5ug immunoprecipitates TPO receptor from 500ug of DAMI RIPA lysate.
Optimal dilutions to be determined by the researcher.
Western Blot (WB)
(Western Blot of DAMI cell lysate using MBS617444 (0.5ug/ml).)
Western Blot (WB)
(Western Blot of DAMI cell lysate using MBS617444 (0.5ug/ml).)
NCBI and Uniprot Product Information
NCBI Description
In 1990 an oncogene, v-mpl, was identified from the murine myeloproliferative leukemia virus that was capable of immortalizing bone marrow hematopoietic cells from different lineages. In 1992 the human homologue, named, c-mpl, was cloned. Sequence data revealed that c-mpl encoded a protein that was homologous with members of the hematopoietic receptor superfamily. Presence of anti-sense oligodeoxynucleotides of c-mpl inhibited megakaryocyte colony formation. The ligand for c-mpl, thrombopoietin, was cloned in 1994. Thrombopoietin was shown to be the major regulator of megakaryocytopoiesis and platelet formation. The protein encoded by the c-mpl gene, CD110, is a 635 amino acid transmembrane domain, with two extracellular cytokine receptor domains and two intracellular cytokine receptor box motifs . TPO-R deficient mice were severely thrombocytopenic, emphasizing the important role of CD110 and thrombopoietin in megakaryocyte and platelet formation. Upon binding of thrombopoietin CD110 is dimerized and the JAK family of non-receptor tyrosine kinases, as well as the STAT family, the MAPK family, the adaptor protein Shc and the receptors themselves become tyrosine phosphorylated. [provided by RefSeq]
Uniprot Description
MPL: a hematopoietic receptor for thrombopoietin. Thrombopoietin is the major regulator of megakaryocytopoiesis and platelet formation. Dimerizes upon binding of thrombopoietin, leading to its phosphorylation and activation of JAKs and STATs. May represent a regulatory molecule specific for TPO-R-dependent immune responses. Two splice-variant isoforms have been described.
Protein type: Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 1p34
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; transmembrane receptor activity
Biological Process: cell proliferation; platelet activation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; homeostasis of number of cells; blood coagulation; regulation of chemokine production
Disease: Myelofibrosis; Amegakaryocytic Thrombocytopenia, Congenital; Thrombocythemia 2
Research Articles on MPL
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Product Notes
The MPL mpl (Catalog #AAA617444) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Thrombopoietin Receptor (c-Mpl) (TPO) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Thrombopoietin Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). WB: 0.25-1ug/ml detects TPO receptor in RIPA lysates from DAMI cells. DAMI cell lysate was resolved by electrophoresis, transferred to PVDF and probed with MBS617444 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system. IHC: Stains megakaryocytes in bone marrow biopsy sections. IP: 5ug immunoprecipitates TPO receptor from 500ug of DAMI RIPA lysate. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the MPL mpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Thrombopoietin Receptor, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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