Goat anti-Human NFIL3 Polyclonal Antibody | anti-NFIL3 antibody
NFIL3 (E4 Promoter Binding Protein 4, E4BP4, E4BP4 Protein, Interleukin 3 Binding Protein 1, IL3BP1, Interleukin 3 Promoter Transcriptional Activator, NFIL3A, Nuclear Factor Interleukin 3 Regulated, Transcriptional Activator NF-IL3A)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: ELISA: 1:8000
Western Blot: ~0.5-1ug/ml. 5~5-60kD band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kD according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2609-14.; PMID: 9122243).
NCBI and Uniprot Product Information
NCBI Description
Expression of interleukin-3 (IL3; MIM 147740) is restricted to activated T cells, natural killer (NK) cells, and mast cell lines. Transcription initiation depends on the activating capacity of specific protein factors, such as NFIL3, that bind to regulatory regions of the gene, usually upstream of the transcription start site (Zhang et al., 1995 [PubMed 7565758]).[supplied by OMIM]
Uniprot Description
E4BP4: Acts as a transcriptional regulator that recognizes and binds to the sequence 5'-[GA]TTA[CT]GTAA[CT]-3', a sequence present in many cellular and viral promoters. Represses transcription from promoters with activating transcription factor (ATF) sites. Represses promoter activity in osteoblasts. Represses transcriptional activity of PER1. Represses transcriptional activity of PER2 via the B- site on the promoter. Activates transcription from the interleukin-3 promoter in T-cells. Competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). Component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. Protects pro-B cells from programmed cell death. Belongs to the bZIP family. NFIL3 subfamily.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 9q22
Cellular Component: nucleus
Molecular Function: DNA binding; transcription factor activity; transcription corepressor activity
Biological Process: transcription from RNA polymerase II promoter; circadian rhythm; immune response; negative regulation of transcription from RNA polymerase II promoter
Research Articles on NFIL3
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Product Notes
The NFIL3 nfil3 (Catalog #AAA617327) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The NFIL3 (E4 Promoter Binding Protein 4, E4BP4, E4BP4 Protein, Interleukin 3 Binding Protein 1, IL3BP1, Interleukin 3 Promoter Transcriptional Activator, NFIL3A, Nuclear Factor Interleukin 3 Regulated, Transcriptional Activator NF-IL3A) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NFIL3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot. Dilution: ELISA: 1:8000 Western Blot: ~0.5-1ug/ml. 5~5-60kD band observed in lysates of Jurkat and HepG2 (calculated MW of 51.5kD according to NP_005375.2). The observed molecular weight corresponds to earlier findings in literature (Ikushima et al., Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2609-14.; PMID: 9122243). Researchers should empirically determine the suitability of the NFIL3 nfil3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NFIL3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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