Rabbit anti-Human RGS3 Polyclonal Antibody | anti-RGS3 antibody

RGS3 (Regulator of G protein signaling 3)

Cat. Num. AAA616454

• Gene Names: RGS3; C2PA; RGP3; FLJ20370; FLJ31516; FLJ90496; PDZ-RGS3
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RGS3; Polyclonal Antibody; RGS3 (Regulator of G protein signaling 3); Anti -RGS3 (Regulator of G protein signaling 3); anti-RGS3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Reacts with the RGS3 protein. Crossreacts with RGS3T, a N-terminal truncated form of RGS3.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in 0.1% sodium azide. Reconstitute to 1mg/ml by adding 200ul PBS.

• Applicable Applications for anti-RGS3 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Western Blot and Immunoprecipitation.; Dilution: Western Blot: 1:5000
• Immunogen: E. coli-expressed human RGS3 (aa 401-519; corresponding to the C-terminal 119 residues)
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-RGS3 antibody

RGS3 (regulator of G protein signaling 3) is a 519-residue protein that is widely expressed. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase-activating protein (GAP). RGS3 negatively regulates the activation of Gi and Gq. A truncated RGS3 (RGS3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS3T may be localized in the nucleus and RGS3 is found in cytoplasm. Translocation to membrane is important for the RGS proteins to interact with heterotrimeric G proteins.

Product Categories/Family for anti-RGS3 antibody

Antibodies; Abs to G Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 55664618
• NCBI GeneID: 5998
• UniProt Accession #: P49796; Q5VXB8; Q5VXC1; Q5VZ05; Q6ZRM5; Q8IUQ1; Q8NC47; Q8NFN4; Q8NFN5; Q8TD59; A6NHA0; A8K0V1; Q53GP3; Q5VXC0; Q5VZ06; Q6ZV17; Q8NFN6; Q8WV02; Q8WVE9; Q96NV5; Q9NX91
• Molecular Weight: 132,336 Da
• NCBI Official Full Name: regulator of G-protein signaling 3
• NCBI Official Synonym Full Names: regulator of G-protein signaling 3
• NCBI Official Symbol: RGS3
• NCBI Official Synonym Symbols: C2PA; RGP3; FLJ20370; FLJ31516; FLJ90496; PDZ-RGS3
• NCBI Protein Information: regulator of G-protein signaling 3; OTTHUMP00000022812; OTTHUMP00000022813; OTTHUMP00000022814; OTTHUMP00000022815; OTTHUMP00000215341; OTTHUMP00000215342; OTTHUMP00000233952; regulator of G-protein signalling 3
• UniProt Protein Name: Regulator of G-protein signaling 3
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS3
• UniProt Entry Name: RGS3_HUMAN

NCBI Description

This gene encodes a member of the regulator of G-protein signaling (RGS) family. This protein is a GTP-ase activating protein which inhibits G-protein mediated signal transduction. The protein is largely cytosolic, but G-protein activation leads to translocation of this protein to the plasma membrane. A nuclear form of this protein has also been described, but its sequence has not been identified. Multiple alternatively spliced transcript variants have been described for this gene but the full-length nature of some transcripts is not yet known. [provided by RefSeq]

Uniprot Description

RGS3: Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G- protein-mediated release of inositol phosphates and activation of MAP kinases. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 9q32

Cellular Component: nucleoplasm; cytoplasm; plasma membrane; cytosol

Molecular Function: GTPase activator activity

Biological Process: regulation of G-protein coupled receptor protein signaling pathway; inactivation of MAPK activity; positive regulation of GTPase activity