Immunofluorescence (IF)
(Confocal microscopy on immunofluorescently detected cytospinned-HL60 cell line. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using MBS616231)
Rabbit anti-Human, Rat Atg16L1 Polyclonal Antibody | anti-ATG16L1 antibody
Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1)
Serum
Serum
Dilution: Immunofluorescence: 1:100-1:3000
Immunohistochemistry: 1:100-1:3000
Western Blot: 1:100-1:3000
Immunofluorescence (IF)
(Confocal microscopy on immunofluorescently detected cytospinned-HL60 cell line. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using MBS616231)
Immunofluorescence (IF)
(Confocal microscopy on immunofluorescently detected cytospinned-HL60 cell line. HL60 cells were fixed with acetone 90 seconds; primary antibody was diluted 1:100 and incubated for 1 hour, 3 x washes and secondary (Goat anti Rb 1:100 alexa 546 conjugate) for 30 minutes. Eosinophils seem to be stained using MBS616231)
Western Blot (WB)
(Western blot (non-reducing) on Jurkat cell lysate using MBS616231)
Western Blot (WB)
(Western blot (non-reducing) on Jurkat cell lysate using MBS616231)
Western Blot (WB)
(Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using MBS616231 at a dilution of 1: 100 (ECL).)
Western Blot (WB)
(Western blot on Peripheral Blood Mononuclear Cells (PMBC) lysate using MBS616231 at a dilution of 1: 100 (ECL).)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.
Uniprot Description
ATG16: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing.
Protein type: Adaptor/scaffold; Membrane protein, peripheral; Autophagy
Chromosomal Location of Human Ortholog: 2q37.1
Cellular Component: pre-autophagosomal structure membrane; autophagic vacuole; axoneme
Molecular Function: identical protein binding; protein binding
Biological Process: protein transport; protein homooligomerization; autophagic vacuole formation
Disease: Inflammatory Bowel Disease 10
Research Articles on ATG16L1
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Product Notes
The ATG16L1 atg16l1 (Catalog #AAA616231) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Atg16L1 (Autophagy-related Protein 16-1, ATG16 Autophagy Related 16-like 1, APG16-like 1, APG16 Autophagy 16-like 1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Atg16L1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunofluorescence: 1:100-1:3000 Immunohistochemistry: 1:100-1:3000 Western Blot: 1:100-1:3000. Researchers should empirically determine the suitability of the ATG16L1 atg16l1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Atg16L1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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