Mouse anti-Human Nuclear Factor I/C Monoclonal Antibody | anti-NFIC antibody

Nuclear Factor I/C (NFIC, CCAAT-binding Transcription Factor, CTF, Nuclear Factor 1 C-Type, CTF5, MGC20153, NF-I, NF1-C, CTF, TGGCA-Binding Protein, NFI) (PE)

Cat. Num. AAA6159186

• Gene Names: NFIC; CTF; NFI; CTF5; NF-I
• Reactivity: Human
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Nuclear Factor I/C; Monoclonal Antibody; Nuclear Factor I/C (NFIC; CCAAT-binding Transcription Factor; CTF; Nuclear Factor 1 C-Type; CTF5; MGC20153; NF-I; NF1-C; TGGCA-Binding Protein; NFI) (PE); anti-NFIC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1C12-2A2
• Specificity: Recognizes human NFIC.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-NFIC antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-429 from human NFIC (AAH12120) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MYSSPLCLTQDEFHPFIEALLPHVRAFAYTWFNLQARKRKYFKKHEKRMSKDEERAVKDELLGEKPEVKQKWASRLLAKLRKDIRPECREDFVLSITGKKAPGCVLSNPDQKGKMRRIDCLRQADKVWRLDLVMVILFKGIPLESTDGERLVKAAQCGHPVLCVQPHHIGVAVKELDLYLAYFVRERDAEQSGSPRTGMGSDQEDSKPITLDTTDFQESFVTSGVFSVTELIQVSRTPVVTGTGPNFSLGELQGHLAYDLNPASTGLRRTLPSTSSSGSKRHKSGSMEEDVDTSPGGDYYTSPSSPTSSCRNWTEDMEGGISSPVKKTEMDKSPFNSPSPQDSPRLSSFTQHHRPVIAVHSGIARSPHPSSALHFPTTSILPQTASTYFPHTAIRYPPHLNPQDPLKDLVSLACDPASQQPGPSWYLG
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (72.82kD).)

Western Blot (WB)

(NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)

Western Blot (WB)

(Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody. Lane 1: NFIC transfected lysate (47.6kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])

Western Blot (WB)

(Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Product Categories/Family for anti-NFIC antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 15082409
• NCBI GeneID: 4782
• Molecular Weight: 48,894 Da
• NCBI Official Full Name: Homo sapiens nuclear factor I/C (CCAAT-binding transcription factor), mRNA
• NCBI Official Synonym Full Names: nuclear factor I/C (CCAAT-binding transcription factor)
• NCBI Official Symbol: NFIC
• NCBI Official Synonym Symbols: CTF; NFI; CTF5; NF-I
• NCBI Protein Information: nuclear factor 1 C-type; CCAAT-box-binding transcription factor; NF-I/C; NF1-C; TGGCA-binding protein
• Protein Family: Nuclear factor

NCBI Description

The protein encoded by this gene belongs to the CTF/NF-I family. These are dimeric DNA-binding proteins, and function as cellular transcription factors and as replication factors for adenovirus DNA replication. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Oct 2011]