Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Mouse anti-Human MYLK2 Monoclonal Antibody | anti-MYLK2 antibody
MYLK2 (Myosin Light Chain Kinase 2 Skeletal/Cardiac Muscle, MLCK2, MLCK, Myosin Light Chain Kinase 2, skMLCK, KMLC) (PE)
Gene Names
MYLK2; KMLC; MLCK; MLCK2; skMLCK
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
MYLK2; Monoclonal Antibody; MYLK2 (Myosin Light Chain Kinase 2 Skeletal/Cardiac Muscle; MLCK2; MLCK; Myosin Light Chain Kinase 2; skMLCK; KMLC) (PE); anti-MYLK2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2G1
Specificity
Recognizes human MYLK2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-MYLK2 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Partial recombinant corresponding to aa161-260 from human MYLK2 (NP_149109) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
KLLAKKPPSEASELTFEGVPMTHSPTDPRPAKAEEGKNILAESQKEVGEKTPGQAGQAKMQGDTSRGIEFQAVPSEKSEVGQALCLTAREEDCFQILDDC
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37kD).)
Western Blot (WB)
(Western Blot analysis of MYLK2 expression in transfected 293T cell line by MYLK2 monoclonal antibody. Lane1: MYLK2 transfected lysate (64.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of MYLK2 expression in transfected 293T cell line by MYLK2 monoclonal antibody. Lane1: MYLK2 transfected lysate (64.7kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western blot analysis of MYLK2 over-expressed 293 cell line, cotransfected with MYLK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MYLK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Western Blot (WB)
(Western blot analysis of MYLK2 over-expressed 293 cell line, cotransfected with MYLK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MYLK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Related Product Information for anti-MYLK2 antibody
This gene encodes a myosin light chain kinase, a calcium/calmodulin dependent enzyme, that is exclusively expressed in adult skeletal muscle.
Product Categories/Family for anti-MYLK2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
64,685 Da
NCBI Official Full Name
myosin light chain kinase 2, skeletal/cardiac muscle
NCBI Official Synonym Full Names
myosin light chain kinase 2
NCBI Official Symbol
MYLK2
NCBI Official Synonym Symbols
KMLC; MLCK; MLCK2; skMLCK
NCBI Protein Information
myosin light chain kinase 2, skeletal/cardiac muscle; myosin light chain kinase 2, skeletal muscle
UniProt Protein Name
Myosin light chain kinase 2, skeletal/cardiac muscle
Protein Family
UniProt Gene Name
MYLK2
UniProt Synonym Gene Names
MLCK2
UniProt Entry Name
MYLK2_HUMAN
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Product Notes
The MYLK2 mylk2 (Catalog #AAA6158969) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MYLK2 (Myosin Light Chain Kinase 2 Skeletal/Cardiac Muscle, MLCK2, MLCK, Myosin Light Chain Kinase 2, skMLCK, KMLC) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MYLK2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the MYLK2 mylk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MYLK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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