Mouse anti-Human ETV6 Monoclonal Antibody | anti-ETV6 antibody

ETV6 (ETS-related Protein Tel1, Tel, ETS Translocation Variant 6, Transcription Factor ETV6, TEL, TEL1) (PE)

Cat. Num. AAA6157699

• Gene Names: ETV6; TEL; THC5; TEL/ABL
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ETV6; Monoclonal Antibody; ETV6 (ETS-related Protein Tel1; Tel; ETS Translocation Variant 6; Transcription Factor ETV6; TEL; TEL1) (PE); anti-ETV6 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3B10
• Specificity: Recognizes human ETV6.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ETV6 antibody: ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Sandwich ELISA: The detection limit is ~0.1ng/ml as a capture antibody.; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-452 from human ETV6 (AAH43399) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MSETPAQCSIKQERISYTPPESPVPSYASSTPLHVPVPRALRMEEDSIRLPAHLRLQPIYWSRDDVAQWLKWAENEFSLRPIDSNTFEMNGKALLLLTKEDFRYRSPHSGDVLYELLQHILKQRKPRILFSPFFHPGNSIHTQPEVILHQNHEEDNCVQRTPRPSVDNVHHNPPTIELLHRSRSPITTNHRPSPDPEQRPLRSPLDNMIRRLSPAERAQGPRPHQENNHQESYPLSVSPMENNHCPASSESHPKPSSPRQESTRVIQLMPSPIMHPLILNPRHSVDFKQSRLSEDGLHREGKPINLSHREDLAYMNHIMVSVSPPEEHAMPIGRIADCRLLWDYVYQLLSDSRYENFIRWEDKESKIFRIVDPNGLARLWGNHKNRTNMTYEKMSRALRHYYKLNIIRKEPGQRLLFRFMKTPDEIMSGRTDRLEHLESQELDEQIYQEDEC
• Conjugate: PE
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (75.46kD).)

Western Blot (WB)

(ETV6 monoclonal antibody Western Blot analysis of ETV6 expression in Jurkat.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ETV6 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged ETV6 is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-ETV6 antibody

Antibodies; Transcription Factors

References

1. ETV6 mutations in early immature human T cell leukemias. Van Vlierberghe P, Ambesi-Impiombato A, Perez-Garcia A, Haydu JE, Rigo I, Hadler M, Tosello V, Della Gatta G, Paietta E, Racevskis J, Wiernik PH, Luger SM, Rowe JM, Rue M, Ferrando AA.J Exp Med. 2011 Dec 19;208(13):2571-9. Epub 2011 Dec 12. 2. The ETS Family Member TEL Binds to Nuclear Receptors RAR and RXR and Represses Gene Activation. Meester-Smoor MA, Janssen MJ, Ter Haar WM, van Wely KH, Aarnoudse AJ, van Oord G, van Tilburg GB, Zwarthoff EC.PLoS One. 2011;6(9):e23620. Epub 2011 Sep 16.

NCBI and Uniprot Product Information

• NCBI GI #: 27695075
• NCBI GeneID: 2120
• Molecular Weight: 53,000 Da
• NCBI Official Full Name: Homo sapiens ets variant 6, mRNA
• NCBI Official Synonym Full Names: ETS variant 6
• NCBI Official Symbol: ETV6
• NCBI Official Synonym Symbols: TEL; THC5; TEL/ABL
• NCBI Protein Information: transcription factor ETV6
• Protein Family: Transcription factor

NCBI Description

This gene encodes an ETS family transcription factor. The product of this gene contains two functional domains: a N-terminal pointed (PNT) domain that is involved in protein-protein interactions with itself and other proteins, and a C-terminal DNA-binding domain. Gene knockout studies in mice suggest that it is required for hematopoiesis and maintenance of the developing vascular network. This gene is known to be involved in a large number of chromosomal rearrangements associated with leukemia and congenital fibrosarcoma. [provided by RefSeq, Sep 2008]