Western Blot (WB)
(Western Blot detection against Immunogen (37.99kD).)
Mouse anti-Human AFF4 Monoclonal Antibody | anti-AFF4 antibody
AFF4 (AF5q31, AF5Q31, MCEF, AF4/FMR2 Family Member 4, ALL1-fused Gene from Chromosome 5q31 Protein, Major CDK9 Elongation Factor-associated Protein) (PE)
Applications are based on unconjugated antibody.
Western Blot (WB)
(Western Blot detection against Immunogen (37.99kD).)
Western Blot (WB)
(Western Blot detection against Immunogen (37.99kD).)
Western Blot (WB)
(Western Blot analysis of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody. Lane 1: AFF4 transfected lysate (40.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of AFF4 expression in transfected 293T cell line by AFF4 monoclonal antibody. Lane 1: AFF4 transfected lysate (40.2kD). Lane 2: Non-transfected lysate.)
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to AFF4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1ug/ml])
Immunohistochemistry (IHC)
(Immunoperoxidase of monoclonal antibody to AFF4 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1ug/ml])
Testing Data
(Detection limit for recombinant GST tagged AFF4 is ~0.03ng/ml as a capture antibody.)
Testing Data
(Detection limit for recombinant GST tagged AFF4 is ~0.03ng/ml as a capture antibody.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the AF4 family of transcription factors involved in leukemia. It is a component of the positive transcription elongation factor b (P-TEFb) complex. A chromosomal translocation involving this gene and MLL gene on chromosome 11 is found in infant acute lymphoblastic leukemia with ins(5;11)(q31;q31q23). [provided by RefSeq, Oct 2011]
Uniprot Description
MCEF: a putative transcription factor. Component of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor b (P-TEFb). Genetic fusion of its gene with MLL has been observed in a subset of acute lymphoblastic leukemia patients. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 5q31
Cellular Component: nucleoplasm; transcription elongation factor complex; mitochondrion; nucleolus; nucleus
Molecular Function: protein binding; transcription factor activity
Biological Process: transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; spermatid development
Disease: Chops Syndrome
Research Articles on AFF4
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Product Notes
The AFF4 aff4 (Catalog #AAA6156423) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AFF4 (AF5q31, AF5Q31, MCEF, AF4/FMR2 Family Member 4, ALL1-fused Gene from Chromosome 5q31 Protein, Major CDK9 Elongation Factor-associated Protein) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's AFF4 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB). IHC-P: 1ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the AFF4 aff4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AFF4, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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