Human anti-Human Jo-1 Polyclonal Antibody
Jo-1 (Histidyl t-RNA Synthetase)
Reactivity
Human
Applications
ELISA, Immunoprecipitation, Gel Super Shift Assay
Purity
PurifiedPurified by fractionation of delipidated and defibrinated donor human serum.
Synonyms
Jo-1; Polyclonal Antibody; Jo-1 (Histidyl t-RNA Synthetase); Anti -Jo-1 (Histidyl t-RNA Synthetase); anti-Jo-1 antibody
Host
Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human Jo-1. Specificity is verified by a precipitin line of identity with the CDC-AF reference serum. No reactivity at a dilution of 1:100 when tested against purified SSA, SSB, Scl-70, Sm and Sm/RNP antigens in an ELISA system designed to detect all IgG subclasses.
Purity/Purification
Purified
Purified by fractionation of delipidated and defibrinated donor human serum.
Purified by fractionation of delipidated and defibrinated donor human serum.
Form/Format
Supplied as a lyophilized powder in 0.01M PBS, 0.02% sodium azide. Reconstitute with 2ml sterile ddH2O or sterile 40-50% glycerol, ddH2O.
Applicable Applications for anti-Jo-1 antibody
ELISA (EL/EIA), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation.
Dilution: ELISA: 1:100
Dilution: ELISA: 1:100
Immunogen
Jo-1 Autoantibody derived from donor human plasma.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Jo-1 antibody
Antibodies generated against the nuclear constituents are known as antinuclear antibodies (ANA). This includes autoantibodies directed against the extractable (soluble in physiological buffers) nuclear antigen or ENA. The most prominent of ANAs/ENAs are autoantibodies which binds to ds-DNA, ss-DNA, histones, ribonucleoproteins (RNP) and the SS-A, SS-B, Sm antigens, Jo-1, and Scl-70. Two antibodies, anti-dsDNA and anti-Sm, appear to occur only in SLE. Others occur in a variety of autoimmune and mixed connective tissue diseases.
Product Categories/Family for anti-Jo-1 antibody
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Product Notes
The Jo-1 (Catalog #AAA615611) is an Antibody produced from Human and is intended for research purposes only. The product is available for immediate purchase. The Jo-1 (Histidyl t-RNA Synthetase) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Jo-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunoprecipitation (IP), Gel Shift Assay (GS/EMSA). Suitable for use in ELISA, Immunodiffusion and Immunoprecipitation. Dilution: ELISA: 1:100. Researchers should empirically determine the suitability of the Jo-1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Jo-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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