anti-Rat EP3 Receptor Polyclonal Antibody

Anti - EP3 Receptor

Cat. Num. AAA615355

• Reactivity: Rat
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: EP3 Receptor; Polyclonal Antibody; Anti - EP3 Receptor; anti-EP3 Receptor antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat EP3. Rat control peptide is conserved in all EP3 isoforms that differ at the C-terminus. It is 75% conserved in mouse EP3-beta and -gamma. No significant homology of control peptide exists with either human EP3 or other EP (1, 2 or 4) receptors.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-EP3 Receptor antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA and Western Blot. Other applications not tested.; Dilution: ELISA: 0.5-1ug/ml; Control peptide can be used to coat ELISA plates at 1ug/ml. See E3364-34.; Western Blot: 1-10ug/ml using ECL technique. An antibody made to this epitope has been shown to detect ~ 62kD protein in the kidney (2).; Optimal dilutions to be determined by the researcher.
• Immunogen: 15aa peptide of rat EP3, conjugated to KLH. Location: ~N-terminal, Extracellular.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-EP3 Receptor antibody

Prostanoids are the cyclooxygenase metabolites derived from C-20 unsaturated fatty acids (arachidonic acid) and include prostaglandin (PG) D2, PGE2, PGF2 alpha, PGI2, and thrombaxane (Tx) A2. Prostaglandin PGE2is one of the major prostaglandin produced during inflammation. A variety of PGE2-mediated effects on vascular smooth muscle tonus, glomerular cell function, renin release, and renal salt and water transport have been described. The actions of PGE2 are mediated by rhodopsin-type; G-protein coupled membrane receptors, termed E-prostanoid (EP) receptors or PTGERs. There are four subtypes of PGE receptors designated as EP1, EP2, EP3, and EP4 that are encoded by different genes and expressed differently in each tissue. The intracellular signaling also differs among the receptor subtypes. In general, EP receptors display a protein topology typical of GPCR - 7 TM domain, an extracellular N-terminus, and a large intracellular C-terminus.

Product Categories/Family for anti-EP3 Receptor antibody

Abs to Prostaglandins

References

• 1. Sugimoto Y et al (1992) J. Biol. Chem. 267:6463.
• 2. Sugimoto Y et al (1993) J. Biol. Chem. 268:2712.
• 3. Irie A et al (1993) Eur. J. Biochem. 217:313.
• 4. Hasegawa H et al (1993) J. Biol. Chem. 271:1857.
• 5. Narumiya S et al (1999) Physiol. Rev. 79:1193 (review).
• 6. Morath R et al (1999) J Am. Soc. Nephrol. 10:1860.
• 7. Walch L 2003, Endocrinology, 144:1284-1291, WB.
• 8. Aronff DM, 2004, J. Immunol., Jul 2004; 173:559 -565, WB.
• 9. Biswas S 2004, Prostaglandins, Leukotrienes and Essential Fatty Acids, 71:277-278, IHC.
• 10. White ES, 2004, Am. J. Respir. Cell Mol. Biol, WB. 11. Aronoff DM, 2004, J. Immunol.,173:559 - 565, WB.