anti-Human Aquaporin, Adipose Specific Polyclonal Antibody | anti-Aqp7 antibody

Anti - Aquaporin, Adipose Specific (AQPAP, AQP7-Like)

Cat. Num. AAA615298

• Gene Names: Aqp7; AQP7L; AQPap
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by immunoaffinity chromatography.
• Synonyms: Aquaporin; Adipose Specific; Polyclonal Antibody; Anti - Aquaporin; Adipose Specific (AQPAP; AQP7-Like); anti-Aqp7 antibody

• Host: Host: Rabbit; Source: Human
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes Aquaporin, adipose specific. The peptide sequence is unique to Aquaporin, adipose specific with no significant homology to any other Aquaporins. Species Crossreactivity: Human.
• Purity/Purification: Purified by immunoaffinity chromatography.
• Form/Format: Affinity Purified; Supplied as a liquid in 100mM Tris, pH 7.4, 0.2% BSA, 0.1% sodium azide, beforet he addition of glycerol to 40%.
• Concentration: ~1mg/ml (varies by lot)
• Sequence Length: 303

• Applicable Applications for anti-Aqp7 antibody: ELISA, Western Blot
• Application Notes: Suitable for use in ELISA, Western Blot. Other applications not tested.; Dilution: Western Blot:1-10ug/ml (ECL). Note: Recommend the use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations in order to suppress non-specific bands.; ELISA: 1:50,000-1:100,000. Control peptide can be used to coat ELISA plates at 1-10ug/ml.; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide 20aa sequence within the N-terminal domain of human Aquaporin, adipose specific (KLH coupled). This domain is predicted to be cytoplasmic.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Aqp7 antibody

Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. AQP0 or MIP26 (major intrinsic protein 26kD), and Aquaporin-1 (AQP1, purified from red cells) also called CHIP-28 (channel forming integral protein, 28kD; 268aa; gene locus 7p14) has been the foundation of the growing family of aquaporin. The lens specific AQP0 represents up to 80% of total lens membrane protein. Defects in MIP26 are cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an amino acid substitution that inhibits targeting of MIP to the cell membrane. Most recently, adipose specific AQP-adipose (342aa; AQPAP or AQP7-like) has been cloned. It facilities water and glycerol transport. AQP families of proteins are predicted to contain six transmembrane domains. The N- and C-terminus are predicted to be cytoplasmic.

Product Categories/Family for anti-Aqp7 antibody

Abs to Aquaporin

References

• 1. Kuriyama, H., et al., BBRC 241: 53-58 (1997).

NCBI and Uniprot Product Information

• NCBI GI #: 15787883
• NCBI GeneID: 11832
• UniProt Accession #: O54794; Q8C630; Q5DX24
• Molecular Weight: 32,667 Da
• NCBI Official Full Name: aquaporin adipose
• NCBI Official Synonym Full Names: aquaporin 7
• NCBI Official Symbol: Aqp7
• NCBI Official Synonym Symbols: AQP7L; AQPap
• NCBI Protein Information: aquaporin-7; AQP-7; OTTMUSP00000006986; OTTMUSP00000006987; aquaglyceroporin-7
• UniProt Protein Name: Aquaporin-7
• Protein Family: Aquaporin
• UniProt Gene Name: Aqp7
• UniProt Entry Name: AQP7_MOUSE