Mouse anti-Human CLDN1 Monoclonal Antibody | anti-CLDN1 antibody

CLDN1 (Claudin-1, Senescence-associated Epithelial Membrane Protein, CLD1, SEMP1, UNQ481/PRO944) (HRP)

Cat. Num. AAA6151836

• Gene Names: CLDN1; CLD1; SEMP1; ILVASC
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Immunoprecipitation, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CLDN1; Monoclonal Antibody; CLDN1 (Claudin-1; Senescence-associated Epithelial Membrane Protein; CLD1; SEMP1; UNQ481/PRO944) (HRP); anti-CLDN1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1C5-D9
• Specificity: Recognizes human CLDN1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-CLDN1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Immunoprecipitation (IP), Western Blot (WB)
• Application Notes: IHC-P: 3-5ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-212 from CLDN1 (AAH12471) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(CLDN1 monoclonal antibody Western Blot analysis of CLDN1 expression in PRC/PRF/5.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to CLDN1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 5ug/ml])

Immunoprecipitation (IP)

(Immunoprecipitation of CLDN1 transfected lysate using CLDN1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CLDN1 rabbit polyclonal antibody.)

Testing Data

(Detection limit for recombinant GST tagged CLDN1 is ~0.03ng/ml as a capture antibody.)

Product Categories/Family for anti-CLDN1 antibody

Antibodies; Claudin

References

1. Epigenetically coordinated GATA2 binding is necessary for endothelium-specific endomucin expression. Kanki Y, Kohro T, Jiang S, Tsutsumi S, Mimura I, Suehiro J, Wada Y, Ohta Y, Ihara S, Iwanari H, Naito M, Hamakubo T, Aburatani H, Kodama T, Minami T.EMBO J. 2011 Jun 10;30(13):2582-95. doi: 10.1038/emboj.2011.173. 2. Cigarette smoking reprograms apical junctional complex molecular architecture in the human airway epithelium in vivo. Shaykhiev R, Otaki F, Bonsu P, Dang DT, Teater M, Strulovici-Barel Y, Salit J, Harvey BG, Crystal RG.Cell Mol Life Sci. 2010 Sep 6. 3. Tumor-Associated Calcium Signal Transducer 2 Is Required for the Proper Subcellular Localization of Claudin 1 and 7. Implications in the Pathogenesis of Gelatinous Drop-Like Corneal Dystrophy. Nakatsukasa M, Kawasaki S, Yamasaki K, Fukuoka H, Matsuda A, Tsujikawa M, Tanioka H, Nagata-Takaoka M, Hamuro J, Kinoshita S.Am J Pathol. 2010 Jul 22. 4. Novel HCV replication mouse model using human hepatocellular carcinoma xenografts. Guevin C, Lamarre A, Labonte P.Antiviral Res. 2009 Oct;84(1):14-22. Epub 2009 Jul 19. 5. Three-dimensional Huh7 cell culture system for the study of Hepatitis C virus infection. Sainz B Jr, TenCate V, Uprichard SL.Virol J. 2009 Jul 15;6:103. 6. Hepatitis C virus association with peripheral blood B lymphocytes potentiates viral infection of liver-derived hepatoma cells. Stamataki Z, Shannon-Lowe C, Shaw J, Mutimer D, Rickinson AB, Gordon J, Adams DH, Balfe P, McKeating JA.Blood. 2009 Jan 15;113(3):585-93. Epub 2008 Oct 6. 7. Protein Kinase A-Dependent Step(s) in Hepatitis C Virus Entry and Infectivity. Farquhar MJ, Harris HJ, Diskar M, Jones S, Mee CJ, Nielsen SU, Brimacombe CL, Molina S, Toms GL, Maurel P, Howl J, Herberg FW, van Ijzendoorn SC, Balfe P, McKeating JA.J Virol. 2008 Sep;82(17):8797-811. Epub 2008 Jun 25. 8. CD81 and Claudin 1 Coreceptor Association: Role in Hepatitis C Virus Entry. Harris HJ, Farquhar MJ, Mee CJ, Davis C, Reynolds GM, Jennings A, Hu K, Yuan F, Deng H, Hubscher SG, Han JH, Balfe P, McKeating JA.J Virol. 2008 May;82(10):5007-20. Epub 2008 Mar 12. 9. Effect of cell polarization on hepatitis C virus viral entry. Mee CJ, Grove J, Harris HJ, Hu K, Balfe P, McKeating JA.J Virol. 2008 Jan;82(1):461-70. Epub 2007 Oct 24.

NCBI and Uniprot Product Information

• NCBI GI #: 15214677
• NCBI GeneID: 9076
• Molecular Weight: 22,744 Da
• NCBI Official Full Name: Homo sapiens claudin 1, mRNA
• NCBI Official Synonym Full Names: claudin 1
• NCBI Official Symbol: CLDN1
• NCBI Official Synonym Symbols: CLD1; SEMP1; ILVASC
• NCBI Protein Information: claudin-1
• Protein Family: Claudin

NCBI Description

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]