Mouse anti-Human AKR1A1 Monoclonal Antibody | anti-AKR1A1 antibody

AKR1A1 (Alcohol Dehydrogenase [NADP+], Aldehyde Reductase, Aldo-keto Reductase Family 1 Member A1, ALDR1, ALR, MGC12529, MGC1380) (HRP)

Cat. Num. AAA6151172

• Gene Names: AKR1A1; ALR; ARM; DD3; ALDR1; HEL-S-6
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AKR1A1; Monoclonal Antibody; AKR1A1 (Alcohol Dehydrogenase [NADP+]; Aldehyde Reductase; Aldo-keto Reductase Family 1 Member A1; ALDR1; ALR; MGC12529; MGC1380) (HRP); anti-AKR1A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1A11-2A4
• Specificity: Recognizes human AKR1A1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-AKR1A1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 0.75ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-325 from AKR1A1 (AAH00670) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MAASCVLLHTGQKMPLIGLGTWKSEPGQVKAAVKYALSVGYRHIDCAAIYGNEPEIGEALKEDVGPGKAVPREELFVTSKLWNTKHHPEDVEPALRKTLADLQLEYLDLYLMHWPYAFERGDNPFPKNADGTICYDSTHYKETWKALEALVAKGLVQALGLSNFNSRQIDDILSVASVRPAVLQVECHPYLAQNELIAHCQARGLEVTAYSPLGSSDRAWRDPDEPVLLEEPVVLALAEKYGRSPAQILLRWQVQRKVICIPKSITPSRILQNIKVFDFTFSPEEMKQLNALNKNWRYIVPMLTVDGKRVPRDAGHPLYPFNDPY
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (61.86kD).)

Western Blot (WB)

(AKR1A1 monoclonal antibody Western Blot analysis of AKR1A1 expression in HL-60.)

Western Blot (WB)

(Western Blot analysis of AKR1A1 expression in transfected 293T cell line by AKR1A1 monoclonal antibody. Lane 1: AKR1A1 transfected lysate (36.6kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to AKR1A1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.75ug/ml])

Testing Data

(Detection limit for recombinant GST tagged AKR1A1 is ~0.1ng/ml as a capture antibody.)

Product Categories/Family for anti-AKR1A1 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 33875727
• NCBI GeneID: 10327
• Molecular Weight: 36,573 Da
• NCBI Official Full Name: Homo sapiens aldo-keto reductase family 1, member A1 (aldehyde reductase), mRNA
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member A1
• NCBI Official Symbol: AKR1A1
• NCBI Official Synonym Symbols: ALR; ARM; DD3; ALDR1; HEL-S-6
• NCBI Protein Information: alcohol dehydrogenase [NADP(+)]
• Protein Family: Alcohol dehydrogenase

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member, also known as aldehyde reductase, is involved in the reduction of biogenic and xenobiotic aldehydes and is present in virtually every tissue. Multiple alternatively spliced transcript variants of this gene exist, all encoding the same protein. [provided by RefSeq, Jan 2011]