Rabbit anti-Human APG5 Polyclonal Antibody | anti-ATG5 antibody

APG5 (APG5 Autophagy 5-like, APG5-like, APG5L, Apoptosis-specific Protein, ASP, ATG5 Autophagy-related 5 Homolog, Autophagy Protein 5, hAPG5, Homolog of S Cerevisiae Autophagy 5)

Cat. Num. AAA614980

• Gene Names: ATG5; ASP; APG5; APG5L; hAPG5; APG5-LIKE
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: APG5; Polyclonal Antibody; APG5 (APG5 Autophagy 5-like; APG5-like; APG5L; Apoptosis-specific Protein; ASP; ATG5 Autophagy-related 5 Homolog; Autophagy Protein 5; hAPG5; Homolog of S Cerevisiae Autophagy 5); Anti -APG5 (APG5 Autophagy 5-like; anti-ATG5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IHC and Western Blot confirmed the specificity for APG5. Species Crossreactivity: Human
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Reconstitute in 500 ul of sterile water. Centrifuge to remove any insoluble material.

• Applicable Applications for anti-ATG5 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
• Immunogen: A synthetic peptide corresponding to the C-terminal of human APG-5L. No immunogenic carrier protein was conjugated to the immunogen. Instead, Adjukine B (see the Adjuvants in biosensis' product list) has been used to orchestrate/boost the immune response.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ATG5 antibody

Autophagy is an important mechanism for nonselective intracellular breakdown whereby cytosol and organelles are encapsulated in vesicles, which are then engulfed and digested by lytic vacuoles/lysosomes. ATG5 is required for autophagy. It conjugates to ATG12 and associates with isolation membrane to form autophagosomes. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. Conjugation to ATG12 is essential for autophagy, but is not required for association with isolation membrane. ATG5 also plays an important role in the apoptotic process. Its expression is a relatively late event in the apoptotic process, occurring downstream of caspase activity.

Product Categories/Family for anti-ATG5 antibody

Antibodies; Abs to Autophagy

NCBI and Uniprot Product Information

• NCBI GI #: 4757798
• NCBI GeneID: 9474
• NCBI Accession #: NP_004840.1
• NCBI GenBank Nucleotide #: NM_004849.2
• UniProt Accession #: Q9H1Y0; O60875; Q5JVR2; Q68DI4; Q9H2B8; Q9HCZ7; Q7Z3H3
• Molecular Weight: 32,447 Da
• NCBI Official Full Name: autophagy protein 5
• NCBI Official Synonym Full Names: ATG5 autophagy related 5 homolog (S. cerevisiae)
• NCBI Official Symbol: ATG5
• NCBI Official Synonym Symbols: ASP; APG5; APG5L; hAPG5; APG5-LIKE
• NCBI Protein Information: autophagy protein 5; OTTHUMP00000017823; OTTHUMP00000017824; OTTHUMP00000017825; apoptosis specific protein; apoptosis-specific protein
• UniProt Protein Name: Autophagy protein 5
• Protein Family: Autophagy protein
• UniProt Gene Name: ATG5
• UniProt Synonym Gene Names: APG5L; ASP
• UniProt Entry Name: ATG5_HUMAN

Uniprot Description

ATG5: Required for autophagy. Conjugates to ATG12 and associates with isolation membrane to form cup-shaped isolation membrane and autophagosome. The conjugate detaches from the membrane immediately before or after autophagosome formation is completed. The ATG5-ATG12 conjugate forms a complex with several units of ATG16. Interacts with TECPR1; the interaction is direct and does not take place when ATG16 is associated with the ATG5- ATG12 conjugate. By apoptotic stimuli. Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells. Belongs to the ATG5 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system; Autophagy; Apoptosis

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: membrane; pre-autophagosomal structure membrane; cytoplasm; autophagic vacuole; axoneme

Molecular Function: protein binding; APG8 conjugating enzyme activity

Biological Process: response to drug; response to fungus; apoptosis; ventricular cardiac muscle cell development; C-terminal protein lipidation; post-translational protein modification; vasodilation; regulation of release of sequestered calcium ion into cytosol; cellular response to nitrogen starvation; heart contraction; regulation of cytokine secretion during immune response; mitochondrion degradation; innate immune response; autophagy; blood vessel remodeling; negative regulation of protein ubiquitination; negative regulation of interferon type I production; otolith development; negative regulation of apoptosis; autophagic vacuole formation