Mouse anti-Human SH3GLB1 Monoclonal Antibody | anti-SH3GLB1 antibody

SH3GLB1 (Endophilin-B1, Bax-interacting Factor 1, Bif-1, SH3 Domain-containing GRB2-like Protein B1, KIAA0491, CGI-61) (FITC)

Cat. Num. AAA6149644

• Gene Names: SH3GLB1; Bif-1; CGI-61; PPP1R70; dJ612B15.2
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SH3GLB1; Monoclonal Antibody; SH3GLB1 (Endophilin-B1; Bax-interacting Factor 1; Bif-1; SH3 Domain-containing GRB2-like Protein B1; KIAA0491; CGI-61) (FITC); anti-SH3GLB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1B3-A5
• Specificity: Recognizes human SH3GLB1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
• Sequence Length: 1244

• Applicable Applications for anti-SH3GLB1 antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Full length recombinant corresponding to aa1-366 from human SH3GLB1 (AAH07455) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLN
• Conjugate: FITC
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (66.26kD).)

Western Blot (WB)

(SH3GLB1 monoclonal antibody, Western Blot analysis of SH3GLB1 expression in K-562.)

Western Blot (WB)

(Western Blot analysis of SH3GLB1 expression in transfected 293T cell line by SH3GLB1 monoclonal antibody. Lane 1: SH3GLB1 transfected lysate (40.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to SH3GLB1 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 3ug/ml])

Testing Data

(Detection limit for recombinant GST tagged SH3GLB1 is ~0.3ng/ml as a capture antibody.)

Product Categories/Family for anti-SH3GLB1 antibody

Antibodies; Apoptosis

NCBI and Uniprot Product Information

• NCBI GI #: 34782815
• NCBI GeneID: 51100
• UniProt Accession #: Q9Y371
• NCBI Official Full Name: Homo sapiens SH3-domain GRB2-like endophilin B1, mRNA
• NCBI Official Synonym Full Names: SH3 domain containing GRB2 like, endophilin B1
• NCBI Official Symbol: SH3GLB1
• NCBI Official Synonym Symbols: Bif-1; CGI-61; PPP1R70; dJ612B15.2
• NCBI Protein Information: endophilin-B1
• UniProt Protein Name: Endophilin-B1
• Protein Family: Endophilin
• UniProt Gene Name: SH3GLB1
• UniProt Synonym Gene Names: KIAA0491; Bif-1
• UniProt Entry Name: SHLB1_HUMAN

NCBI Description

This gene encodes a SRC homology 3 domain-containing protein. The encoded protein interacts with the proapoptotic member of the Bcl-2 family, Bcl-2-associated X protein (Bax) and may be involved in regulating apoptotic signaling pathways. This protein may also be involved in maintaining mitochondrial morphology. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Sep 2011]

Uniprot Description

SH3GLB1: May be required for normal outer mitochondrial membrane dynamics. Required for coatomer-mediated retrograde transport in certain cells. May recruit other proteins to membranes with high curvature. May promote membrane fusion. Binds DNM1, HTT, AMPH, BIN1 and ARFGAP1. Homodimer, and heterodimer with SH3GLB2. Binds BAX. Induction of apoptosis augments BAX binding. Highly expressed in heart, skeletal muscle, kidney and placenta. Detected at lower levels in brain, colon, thymus, spleen, liver, small intestine, lung and peripheral blood leukocytes. Belongs to the endophilin family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 2.3.1.-; Autophagy; Mitochondrial; Transferase; Apoptosis

Chromosomal Location of Human Ortholog: 1p22

Cellular Component: Golgi membrane; mitochondrial outer membrane; cytoplasm

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; lipid binding

Biological Process: positive regulation of protein oligomerization; apoptosis; protein oligomerization