CTGF Antibody | anti-CTGF antibody
CTGF (Connective Tissue Growth Factor, CCN2, Hcs24, Hypertrophic Chondrocyte-specific Gene Product 24, IGFBP8, Insulin-like Growth Factor-Binding Protein 8)
This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive malignant carcinoma. The recommended concentration is 0.125 ug/mL-0.250 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary.
Neutrilization:
To yield one-half maximal inhibition [ND50] of the biological activity of human CTGF (5.0 ug/ml), a concentration of 6.0 - 9.0 ug/ml of this antibody is required.
Sandwich ELISA:
To detect Human CTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (MBS695113) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTGF.
Western Blot:
To detect Human CTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Optimal dilutions should be determined by each laboratory for each application.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis. [provided by RefSeq]
Uniprot Description
CTGF: Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes. Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells. Enhances fibroblast growth factor-induced DNA synthesis. Belongs to the CCN family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell adhesion; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 6q23.1
Cellular Component: Golgi apparatus; proteinaceous extracellular matrix; extracellular space; cis-Golgi network; intracellular membrane-bound organelle; perinuclear region of cytoplasm; extracellular region; plasma membrane; cell cortex; cytosol
Molecular Function: integrin binding; heparin binding; insulin-like growth factor binding; protein binding; growth factor activity; fibronectin binding
Biological Process: response to peptide hormone stimulus; epidermis development; response to anoxia; cell-matrix adhesion; positive regulation of collagen biosynthetic process; positive regulation of JNK cascade; positive regulation of caspase activity; cellular lipid metabolic process; response to estradiol stimulus; cell-cell signaling; positive regulation of cell proliferation; positive regulation of stress fiber formation; response to wounding; response to glucose stimulus; tissue homeostasis; angiogenesis; regulation of cell growth; cell differentiation; cell adhesion; positive regulation of cell activation; cartilage condensation; integrin-mediated signaling pathway; transcription initiation from RNA polymerase II promoter; ossification; cell migration; fibroblast growth factor receptor signaling pathway; organ senescence; response to mineralocorticoid stimulus; response to amino acid stimulus; gene expression; positive regulation of protein amino acid phosphorylation; regulation of chondrocyte differentiation; positive regulation of cell differentiation; lung development
Research Articles on CTGF
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Product Notes
The CTGF ctgf (Catalog #AAA614804) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's CTGF can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), Neutrilization, Sandwich ELISA. Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive malignant carcinoma. The recommended concentration is 0.125 ug/mL-0.250 ug/mL with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Neutrilization: To yield one-half maximal inhibition [ND50] of the biological activity of human CTGF (5.0 ug/ml), a concentration of 6.0 - 9.0 ug/ml of this antibody is required. Sandwich ELISA: To detect Human CTGF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTGF (MBS695113) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant Human CTGF. Western Blot: To detect Human CTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the CTGF ctgf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTGF, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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