Rabbit anti-Human cdc14B Phosphatase Polyclonal Antibody | anti-CDC14B antibody

cdc14B Phosphatase

Cat. Num. AAA614763

• Gene Names: CDC14B; CDC14B3; Cdc14B1; Cdc14B2; hCDC14B
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: cdc14B Phosphatase; Polyclonal Antibody; Anti -cdc14B Phosphatase; anti-CDC14B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes the human Cdc14B protein including isoforms 1, 2 and 3. The calculated molecular weight of Cdc14B isoforms range from 53-60kD. The apparent molecular weight of Cdc14B in HeLa cells was ~66kD. Reactivity is confirmed with human HeLa cell lysates.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-CDC14B antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation and Western Blot.; Dilution: Immunoprecipitation: 1-3ug/ml; Western Blot: 1-3ug/ml
• Immunogen: Synthetic peptide derived from the N-terminal region of the human Cdc14B protein
• Positive Control: Human HeLa cell lysates
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CDC14B antibody

Cdc14, also known as duel specificity phosphatase, is a protein phosphatase originally discovered in yeast that plays an important role in cell cycle regulation, including exit from mitosis (1). Throughout G1, S and G2 phases, Cdc14 is located in the nucleolus; at the beginning of anaphase, the protein is redistributed throughout the entire nucleus (2). In late anaphase, Cdc14 dephosphorylates Cdh1, which then activates APC (Anaphase Promoting Complex) (3).

Product Categories/Family for anti-CDC14B antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 5706724
• NCBI GeneID: 8555
• UniProt Accession #: O60729; O43183; O60730; Q5JU08; A6N5X8; B1AL31; B1AL32; Q5JU06
• Molecular Weight: 56,802 Da
• NCBI Official Full Name: Cdc14B3 phosphatase
• NCBI Official Synonym Full Names: CDC14 cell division cycle 14 homolog B (S. cerevisiae)
• NCBI Official Symbol: CDC14B
• NCBI Official Synonym Symbols: CDC14B3; Cdc14B1; Cdc14B2; hCDC14B
• NCBI Protein Information: dual specificity protein phosphatase CDC14B; OTTHUMP00000021729; OTTHUMP00000021730; OTTHUMP00000021732; OTTHUMP00000215877; OTTHUMP00000215878; OTTHUMP00000215879
• UniProt Protein Name: Dual specificity protein phosphatase CDC14B
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: CDC14B
• UniProt Entry Name: CC14B_HUMAN

NCBI Description

The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq]

Uniprot Description

CDC14B: Dual-specificity phosphatase involved in DNA damage response. Essential regulator of the G2 DNA damage checkpoint: following DNA damage, translocates to the nucleus and dephosphorylates FZR1/CDH1, a key activator of the anaphase promoting complex/cyclosome (APC/C). Dephosphorylation of FZR1/CDH1 activates the APC/C, leading to the ubiquitination of PLK1, preventing entry into mitosis. Preferentially dephosphorylates proteins modified by proline-directed kinases. Belongs to the protein-tyrosine phosphatase family. Non-receptor class CDC14 subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Motility/polarity/chemotaxis; Nucleolus; EC 3.1.3.16; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 9q22.3

Cellular Component: nucleoplasm; nuclear membrane; nucleolus; nucleus

Molecular Function: protein binding; protein tyrosine/serine/threonine phosphatase activity; protein tyrosine phosphatase activity; protein serine/threonine phosphatase activity

Biological Process: anaphase-promoting complex activation; protein amino acid dephosphorylation; G2/M transition DNA damage checkpoint; DNA repair