Chicken Vacuolar Protein Sorting 28 Polyclonal Antibody | anti-VPS28 antibody

Vacuolar Protein Sorting 28 (Yeast) (VVPS 28, VPS28, MGC60323, Tumor Susceptibility Gene 101 TSG101, Yeast Class E Protein Vps28p Homolog)

Cat. Num. AAA614741

• Gene Names: VPS28; MGC60323
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Vacuolar Protein Sorting 28; Polyclonal Antibody; Vacuolar Protein Sorting 28 (Yeast) (VVPS 28; VPS28; MGC60323; Tumor Susceptibility Gene 101 TSG101; Yeast Class E Protein Vps28p Homolog); Anti -Vacuolar Protein Sorting 28 (Yeast) (VVPS 28; anti-VPS28 antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Specificity: Recognizes human VPS28. Species Crossreactivity: Mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Glycerol free.

• Applicable Applications for anti-VPS28 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Synthetic peptide corresponding to aa1-221
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot: E coli-derived fusion protein as test antigen. Affinity-purified IgY dilution: 1:2000, Goat anti-IgY-HRP dilution: 1:1000. Colorimetric method for signal development.)

Related Product Information for anti-VPS28 antibody

This protein is involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. It is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37.

Product Categories/Family for anti-VPS28 antibody

Antibodies; Abs to Cancer Markers

NCBI and Uniprot Product Information

• NCBI GI #: 34452693
• NCBI GeneID: 51160
• NCBI Accession #: NP_898880.1
• NCBI GenBank Nucleotide #: NM_183057.1
• UniProt Accession #: Q86VK0; Q548N1; Q71MH5; Q9UK41
• Molecular Weight: 26,463 Da
• NCBI Official Full Name: vacuolar protein sorting-associated protein 28 homolog isoform 2
• NCBI Official Synonym Full Names: vacuolar protein sorting 28 homolog (S. cerevisiae)
• NCBI Official Symbol: VPS28
• NCBI Official Synonym Symbols: MGC60323
• NCBI Protein Information: vacuolar protein sorting-associated protein 28 homolog; H-Vps28; OTTHUMP00000228917; OTTHUMP00000228919; OTTHUMP00000228927; OTTHUMP00000228928; ESCRT-I complex subunit VPS28; yeast class E protein Vps28p homolog
• UniProt Protein Name: Uncharacterized protein
• Protein Family: Vacuolar protein sorting-associated protein
• UniProt Gene Name: VPS28
• UniProt Entry Name: Q86VK0_HUMAN

NCBI Description

This gene encodes a protein involved in endosomal sorting of cell surface receptors via a multivesicular body/late endosome pathway. The encoded protein is one of the three subunits of the ESCRT-I complex (endosomal complexes required for transport) involved in the sorting of ubiquitinated proteins. The two other subunits of ESCRT-I are vesicular protein sorting 23, also known as tumor susceptibility gene 101 (TSG101), and vesicular protein sorting 37. Two alternative transcripts encoding different isoforms have been described. Additional alternative transcripts may exist but the proteins encoded by these transcripts have not been verified experimentally. [provided by RefSeq]

Uniprot Description

VPS28: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Belongs to the VPS28 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 8q24.3

Cellular Component: late endosome membrane; cytoplasm; plasma membrane; endosome membrane; cytosol; endosome

Molecular Function: protein binding; protein complex binding

Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; viral protein processing; negative regulation of protein ubiquitination; virus assembly; viral infectious cycle; endosome transport; protein targeting to vacuole during ubiquitin-dependent protein catabolic process via the MVB pathway