Rabbit Glycine Transporter-2 Polyclonal Antibody | anti-slc6a5 antibody

Glycine Transporter-2 (GLYT2)

Cat. Num. AAA614492

• Gene Names: slc6a5; glyt2; slc6a5
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Purity: Serum; Serum.
• Synonyms: Glycine Transporter-2; Polyclonal Antibody; Glycine Transporter-2 (GLYT2); Anti -Glycine Transporter-2 (GLYT2); anti-slc6a5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes rat GLYT2. No homology is seen with either GLYT1 or other transporters. Species Sequence Homology
• Purity/Purification: Serum; Serum.
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide.

• Applicable Applications for anti-slc6a5 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000 (ECL).; ELISA: 1:10,000-1:50,000. Coat with 50-100ng control peptide/well.
• Immunogen: Synthetic peptide consisting of 20aa sequence within the cytoplasmic, C-terminal domain of rat GLYT2 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-slc6a5 antibody

Glycine is one of the major inhibitory neurotransmitters in the spinal cord and brain stem. Glycine acts on the strychnine-sensitive glycine receptors, a pentameric Cl-channel, to generate inhibitory postsynaptic potentials. Glycine can modulate excitatory neurotransmission as an obligatory co-agonist with glutamate at NMDA-activated glutamate receptors via binding site on the NMDA receptor, which is distinct from the strychnine-sensitive glycine receptor-binding site. Glycinergic synapses are primarily present throughout the spinal cord, brain stem and cerebellum, as well as in the retina and a few other brain regions. Neurotransmitter action is terminated by re-uptake of glycine via specific high affinity transporter protein located at the plasma membrane of neuron and glial cells. The glycine transporter belongs to a family of Na/Cl-dependent neurotransmitter transporters, predicted to contain as many as 12 transmembrane domains. Glycine receptor exists in 2 forms (GLYT1 and GLYT2). Form 1 has three other known isoform (GLYT1a, GLYT1b and GLYT1c) which may be produced by alternative spicing or promoter usage. Rodent GLYT1a and GLYT1b differ only by 10 amino acid at the N-terminus and expressed from the same gene.

Product Categories/Family for anti-slc6a5 antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 157058891
• NCBI GeneID: 100126617
• UniProt Accession #: A7Y2X0
• Molecular Weight: 87,452 Da
• NCBI Official Full Name: glycine transporter 2
• NCBI Official Synonym Full Names: solute carrier family 6 (neurotransmitter transporter, glycine), member 5
• NCBI Official Symbol: slc6a5
• NCBI Official Synonym Symbols: glyt2; slc6a5
• NCBI Protein Information: sodium- and chloride-dependent glycine transporter 2; glyT-2; xGlyT2; solute carrier family 6 member 5
• UniProt Protein Name: Sodium- and chloride-dependent glycine transporter 2
• Protein Family: Sodium- and chloride-dependent glycine transporter
• UniProt Gene Name: slc6a5
• UniProt Synonym Gene Names: glyt2
• UniProt Entry Name: SC6A5_XENLA

Uniprot Description

Function: Terminates the action of glycine by its high affinity sodium-dependent reuptake into presynaptic terminals. May be responsible for the termination of neurotransmission at strychnine-sensitive glycinergic synapses

By similarity. UniProtKB Q9Y345

Subcellular location: Membrane; Multi-pass membrane protein

Potential.

Tissue specificity: First expressed in late neurula stages in the anterior spinal cord, where expression intensifies through the tailbud stages, and by hatching, expression is seen in the hindbrain. During late hatching stages, expression extends along most of the length of the spinal cord, mildly intensifies in the hindbrain, and appears in localized regions of the lateral forebrain and medial midbrain. By the swimming tadpole stage, weak expression appears in the anterior hindbrain, with stronger expression in the posterior, post-mitotic neurons. Ref.1

Sequence similarities: Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A5 subfamily. [View classification]