Mouse anti-Human Glycophorin C Monoclonal Antibody | anti-GYPC antibody

Glycophorin C (Glycophorin-C, Glycoconnectin, Glycophorin-D, GPD, Glycoprotein beta, PAS-2', Sialoglycoprotein D, CD236, GYPC, GLPC, GPC) (Biotin)

Cat. Num. AAA6142099

• Gene Names: GYPC; GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2'; GE:GPC:GPD:GYPD
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: Glycophorin C; Monoclonal Antibody; Glycophorin C (Glycophorin-C; Glycoconnectin; Glycophorin-D; GPD; Glycoprotein beta; PAS-2'; Sialoglycoprotein D; CD236; GYPC; GLPC; GPC) (Biotin); anti-GYPC antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 1H3
• Specificity: Recognizes human GYPC.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-GYPC antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa1-57 from human GYPC (NP_002092) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: MWSTRSPNSTAWPLSLEPDPGMASASTTMHTTTIAEPDPGMSGWPDGRMETSTPTIM
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (32.01kD).)

Related Product Information for anti-GYPC antibody

Four main species of sialic acid-rich glycoproteins known as glycophorins A, B, C, and D have been characterized on human erythrocytes. These components carry blood group determinants and various receptors for lectins, bacteria, viruses, and parasites. Glycophorin C (GPC) is the minor constituent carrying the Gerbich blood group antigens which plays significant role in the maintenance of erythrocyte shape by regulating the deformability and mechanical stability of red cells. The protein is a single polypeptide chain of 128aa spanning the phospholipid bilayer. The gene encoding for glycophorin C is mapped on 2q14-q21 region of human chromosome organized in four exons over 13.5kb pair DNA and contains two internal direct repeated domains of 3.4kb which are likely to derive from a recent duplication. Glycophorin C has very little homology with glycophorin A and B.

Product Categories/Family for anti-GYPC antibody

Antibodies; CD Markers

NCBI and Uniprot Product Information

• NCBI GI #: 4504229
• NCBI GeneID: 2995
• NCBI Accession #: NP_002092
• NCBI GenBank Nucleotide #: NM_002101
• UniProt Accession #: P04921; Q53SV9; Q92642; B2R522
• Molecular Weight: 7.2kDa (66aa) 18-28KDa (SDS-PAGE under reducing conditions.)
• NCBI Official Full Name: glycophorin-C isoform 1
• NCBI Official Synonym Full Names: glycophorin C (Gerbich blood group)
• NCBI Official Symbol: GYPC
• NCBI Official Synonym Symbols: GE; GPC; GPD; GYPD; CD236; PAS-2; CD236R; PAS-2'; GE:GPC:GPD:GYPD
• NCBI Protein Information: glycophorin-C
• UniProt Protein Name: Glycophorin-C
• Protein Family: Glycophorin
• UniProt Gene Name: GYPC
• UniProt Synonym Gene Names: GLPC; GPC; GPD
• UniProt Entry Name: GLPC_HUMAN

NCBI Description

Glycophorin C (GYPC) is an integral membrane glycoprotein. It is a minor species carried by human erythrocytes, but plays an important role in regulating the mechanical stability of red cells. A number of glycophorin C mutations have been described. The Gerbich and Yus phenotypes are due to deletion of exon 3 and 2, respectively. The Webb and Duch antigens, also known as glycophorin D, result from single point mutations of the glycophorin C gene. The glycophorin C protein has very little homology with glycophorins A and B. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

GYPC: This protein is a minor sialoglycoprotein in human erythrocyte membranes. The blood group Gerbich antigens and receptors for Plasmodium falciparum merozoites are most likely located within the extracellular domain. Glycophorin-C plays an important role in regulating the stability of red cells. Belongs to the glycophorin-C family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q14-q21

Cellular Component: cortical cytoskeleton; membrane; integral to plasma membrane; plasma membrane

Molecular Function: protein binding

Disease: Malaria, Susceptibility To; Blood Group, Gerbich System