Rabbit RSK1, RSK2 Polyclonal Antibody | anti-Rps6ka3 antibody

RSK1, RSK2, phosphorylated (Ser380/Ser386) (Ribosomal S6 Kinase 1, RSK-1, 90kD Ribosomal Protein S6 Kinase 1, p90-RSK 1, p90RSK1, p90S6K, MAP Kinase-activated Protein Kinase 1a, MAPK-activated Protein Kinase 1a, MAPKAP Kinase 1a, MAPKAPK1A, MAPKAPK-1a, Ri

Cat. Num. AAA613844

• Gene Names: Rps6ka3; Rsk2; MPK-9; pp90RSK2; S6K-alpha3
• Reactivity: Chicken, Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: RSK1; RSK2; Polyclonal Antibody; phosphorylated (Ser380/Ser386) (Ribosomal S6 Kinase 1; RSK-1; 90kD Ribosomal Protein S6 Kinase 1; p90-RSK 1; p90RSK1; p90S6K; MAP Kinase-activated Protein Kinase 1a; MAPK-activated Protein Kinase 1a; MAPKAP Kinase 1a; MAPKAPK1A; MAPKAPK-1a; Ri; Anti -RSK1; anti-Rps6ka3 antibody

• Host: Rabbit
• Reactivity: Chicken, Human, Mouse
• Clonality: Polyclonal
• Specificity: Recognizes human, Rsk1 phosphorylated (Ser380/Ser386). Species Crossreactivity
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide, 50% glycerol.

• Applicable Applications for anti-Rps6ka3 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot. Other applications not tested; Dilution: Western Blot: 1:1000; Optimal dilution determined by the researcher.
• Immunogen: Synthetic phosphopeptide derived from a region of human RSK1 that contains serine 380.
• Positive Control: A431 cells treated with 100 ng/mL PMA for 30 minutes at 37 degree C; HEK293E cells transfected with human RSK1, chicken RSK1, mouse RSK2, or human RSK3.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Rps6ka3 antibody

RSK1 (also called MAPK Activated Protein Kinase-1a; MAPKAP-K1a) is a member of the broadly expressed p90 Ribosomal S6 Kinase (RSK) family of serine/threonine kinases that also includes RSK2 (MAPKAP-K1b), RSK3 (MAPKAP-K1c) and RSK4. RSK proteins are substrates for and downstream transducers of, MAPK signaling proteins, primarily ERK1&2. RSK proteins possess two separate kinase domains, the C-terminal kinase domain and the N-terminal kinase domain, separated by a linker region containing a hydrophobic motif. Activation of RSK is a multi-step process involving phosphorylation of multiple residues within the three domains, changes in RSK protein localization and modulation of complex formation with ERK1&2 and/or 14-3-3beta proteins. RSK is activated by many stimuli including growth factors, phorbol esters, cAMP, heat shock and irradiation. ERK1&2 phosphorylate threonine 573 (threonine 577 in RSK2) of the C-terminal kinase domain, thereby stimulating this kinase activity and leading to phosphorylation of serines 363 and 380 (serines 369 and 386 in RSK2) within the linker region. This phosphorylation is required for activation of the N-terminal kinase domain by PDK1. The activated N-terminal kinase domain phosphorylates various substrates that include transporters, transcription factors and transcription co-activators.

Product Categories/Family for anti-Rps6ka3 antibody

Antibodies; Abs to Protein Kinases; Ribosomal

NCBI and Uniprot Product Information

• NCBI GI #: 22507357
• NCBI GeneID: 110651
• NCBI Accession #: NP_683747.1
• NCBI GenBank Nucleotide #: NM_148945.1
• UniProt Accession #: P18654; Q03140; Q8K3J8; B1AXN4
• Molecular Weight: 83,694 Da
• NCBI Official Full Name: ribosomal protein S6 kinase alpha-3
• NCBI Official Synonym Full Names: ribosomal protein S6 kinase polypeptide 3
• NCBI Official Symbol: Rps6ka3
• NCBI Official Synonym Symbols: Rsk2; MPK-9; pp90RSK2; S6K-alpha3
• NCBI Protein Information: ribosomal protein S6 kinase alpha-3; RSK-2; p90RSK3; p90-RSK 3; MAPKAPK-1b; S6K-alpha-3; MAPKAP kinase 1b; ribosomal S6 kinase 2; ribosomal protein S6 kinase 2; MAPK-activated protein kinase 1b; 90 kDa ribosomal protein S6 kinase 3; MAP kinase-activated protein kinase 1b
• UniProt Protein Name: Ribosomal protein S6 kinase alpha-3
• Protein Family: Ribosomal protein S6 kinase
• UniProt Gene Name: Rps6ka3
• UniProt Synonym Gene Names: Mapkapk1b; Rps6ka-rs1; Rsk2; S6K-alpha-3; p90-RSK 3; p90RSK3; MAPK-activated protein kinase 1b; MAPKAP kinase 1b; MAPKAPK-1b; RSK-2
• UniProt Entry Name: KS6A3_MOUSE

Uniprot Description

RSK2: an AGC kinase of the RSK family. Phosphorylated and activated by Erk1 and -2 in response to many growth factors, polypeptide hormones and neurotransmitters. Several phosphorylation sites are important for its activation. Possesses two kinase domains connected by a regulator linker region. Phosphorylates a wide range of substrates including ribosomal protein S6. Prominently expressed in brain structures essential for cognitive function and learning.

Protein type: EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); Protein kinase, AGC; Kinase, protein; AGC group; RSK family; RSK subfamily

Cellular Component: cytoplasm; nucleus

Molecular Function: transferase activity; protein serine/threonine kinase activity; caspase inhibitor activity; transferase activity, transferring phosphorus-containing groups; magnesium ion binding; nucleotide binding; kinase activity; ribosomal protein S6 kinase activity; protein kinase binding; ATP binding; protein kinase activity

Biological Process: regulation of translation; cellular response to stress; toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; negative regulation of caspase activity; cell cycle; phosphorylation; protein amino acid phosphorylation