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Western Blot (WB)
(Western Blot detection against immunogen (73.26kD).)
Mouse anti-Human Keratin 17 Monoclonal Antibody | anti-KRT17 antibody
Keratin 17 (Keratin Type I Cytoskeletal 17, Keratin-17, K17, KRT17, 39.1, Cytokeratin-17, CK-17) APC
Gene Names
KRT17; PC; K17; PC2; 39.1; CK-17; PCHC1
Reactivity
Human
Applications
ELISA, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
Keratin 17; Monoclonal Antibody; Keratin 17 (Keratin Type I Cytoskeletal 17; Keratin-17; K17; KRT17; 39.1; Cytokeratin-17; CK-17) APC; anti-KRT17 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2D4-1G9
Specificity
Recognizes human Keratin 17.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-KRT17 antibody
ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant protein corresponding to aa1-432 of human Keratin 17 (BC000159; AAH00159) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MTTSIRQFTSSSSIKGSSGLGGGSSRTSCRLSGGLGAGSCRLGSAGGLGSTLGGSSYSSCYSFGSGGGYGSSFGGVDGLLAGGEKATMQNLNDRLASYLDKVRALEEANTELEVKIRDWYQRQAPGPARDYSQYYRTIEELQNKILTATVDNANILLQIDNARLAADDFRTKFETEQALRLSVEADINGLRRVLDELTLARADLEMQIENLKEELAYLKKNHEEEMNALRGQVGGEINVEMDAAPGVDLSRIPNEMRDQYEKMAEKNRKDAEDWFFSKTEELNREVATNSELVQSGKSEISELRRTMQALEIELQSQLSMKASLEGNLAETENRYCVQLSQIQGLIGSVEEQLAQLRCEMEQQNQEYKILLDVKTRLEQEIATYRRLLEGEDAHLTQYKKEPVTTRQVRTIVEEVQDGKVISSREQVHQTTR
Conjugate
APC
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western Blot detection against immunogen (73.26kD).)
Western Blot (WB)
(Western Blot detection against immunogen (73.26kD).)
Western Blot (WB)
(Western Blot analysis of KRT17 expression in A-431 using 128772.)
Western Blot (WB)
(Western Blot analysis of KRT17 expression in A-431 using 128772.)
Immunoprecipitation (IP)
(Immunoprecipitation of KRT17 transfected lysate using 128772 and Protein A Magnetic Bead, and immunoblotted with 128772.)
Immunoprecipitation (IP)
(Immunoprecipitation of KRT17 transfected lysate using 128772 and Protein A Magnetic Bead, and immunoblotted with 128772.)
Immunohistochemistry (IHC)
(Immunoperoxidase on formalin-fixed paraffin-embedded human tonsil using 128772 (3ug/ml).)
Immunohistochemistry (IHC)
(Immunoperoxidase on formalin-fixed paraffin-embedded human tonsil using 128772 (3ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence to KRT17 on HeLa cells using 128772 (25ug/ml).)
Immunofluorescence (IF)
(Immunofluorescence to KRT17 on HeLa cells using 128772 (25ug/ml).)
Related Product Information for anti-KRT17 antibody
The type I keratin 17 (K17) shows a peculiar localization in human epithelial appendages including hair follicles, which undergo a growth cycle throughout adult life. Additionally K17 is induced, along with K6 and K16, early after acute injury to human skin. Predominant expression of K17 and the frequent expression of K8 and K19, with little K6/K16 and K1/K10 expression are the characteristic features of basal cell carcinomas (BCC). This expresssion indicates BCC is differentiated towards undifferentiated follicular epithelia, most probably hair bulge cells.
Product Categories/Family for anti-KRT17 antibody
References
1. Novel markers for differentiation of lobular and ductal invasive breast carcinomas by laser microdissection and microarray analysis. Turashvili G, Bouchal J, Baumforth K, Wei W, Dziechciarkova M, Ehrmann J, Klein J, Fridman E, Skarda J, Srovnal J, Hajduch M, Murray P, Kolar Z.BMC Cancer. 2007 Mar 27;7:55.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
52KD
NCBI Official Full Name
Homo sapiens keratin 17, mRNA
NCBI Official Synonym Full Names
keratin 17
NCBI Official Symbol
KRT17
NCBI Official Synonym Symbols
PC; K17; PC2; 39.1; CK-17; PCHC1
NCBI Protein Information
keratin, type I cytoskeletal 17
Protein Family
NCBI Description
This gene encodes the type I intermediate filament chain keratin 17, expressed in nail bed, hair follicle, sebaceous glands, and other epidermal appendages. Mutations in this gene lead to Jackson-Lawler type pachyonychia congenita and steatocystoma multiplex. [provided by RefSeq, Aug 2008]
Research Articles on KRT17
Similar Products
Product Notes
The KRT17 (Catalog #AAA6137282) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Keratin 17 (Keratin Type I Cytoskeletal 17, Keratin-17, K17, KRT17, 39.1, Cytokeratin-17, CK-17) APC reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 17 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the KRT17 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 17, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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