Chicken Ghrelin Polyclonal Antibody | anti-GHRL antibody
Ghrelin
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Ghrelin; Polyclonal Antibody; Anti -Ghrelin; anti-GHRL antibody
Host
Chicken
Reactivity
Bovine, Canine, Human, Mouse, Porcine, Rat
Clonality
Polyclonal
Isotype
IgY
Specificity
Recognizes Ghrelin. Species crossreactivity: The 15-aa human sequence is 100% conserved in mouse, canine, rat, 86% in porcine and 66% in bovine.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in TBS, pH 7.4, 0.4% BSA, 0.02% sodium merthiolate, 40% glycerol.
Applicable Applications for anti-GHRL antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA, Western Blot, Antibody Blocking.
Dilution: Western Blot: 1-10ug/ml using ECL. Full length ghrelin is ~3.5kD.
ELISA: 1:100,000 using 50-100ng control peptide/well.
Antibody Blocking: Use 5-10ug of control peptide per 1ug antibody.
Dilution: Western Blot: 1-10ug/ml using ECL. Full length ghrelin is ~3.5kD.
ELISA: 1:100,000 using 50-100ng control peptide/well.
Antibody Blocking: Use 5-10ug of control peptide per 1ug antibody.
Immunogen
Synthetic peptide (15 aa) from the C-terminus of mature human ghrelin was coupled to KLH (1).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-GHRL antibody
Growth hormone (GH), produced and released from the anterior pituitary, controls body growth, carbohydrate-protein-lipid metabolism and water-electrolyte balance. GH secretagogues (GHSs) are synthetic compounds that are potent stimulators of GH release. GHSs, act through a novel orphan G-protein-coupled receptor (GPCR), the GHS receptor (GHS-R). Ghrelin ('ghre' is the Proto-Indo-European root of the word "grow") has been purified and identified from rat and human stomach as the endogenous ligand for the GHS-R. The rat and human mature Ghrelin (28-aa) are produced from 117 amino acids precursor. In rat stomach, a 28-aa Ghrelin and des-Gln14-Ghrelin (deletion of Gln14) are produced due to alternative splicing of Ghrelin mRNA. The activity of both Ghrelins is the same. However, des-Gln14-Ghrelin is only present in low amounts in the stomach, indicating that Ghrelin is the major active form. Ghrelin has an unusual modification at Ser3 residue that is n-octanoylated and it is essential for biological activity. Ghrelin is the first known example of a bioactive peptide modified by an acyl acid.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake. Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects. Ghrelin administration stimulates GH secretion but also causes weight gain by increasing food intake and reduction in fat utilization and anti-Ghrelin IgG administration suppressed feeding.
Rat Ghrelin is expressed in the stomach, small and large intestines, and brain regions (hypothalamic arcuate nucleus) that are involved the regulation of food intake. Both Ghrelin and GHS-R expression is detected in the heart, suggesting that Ghrelin might have some cardiovascular effects. Ghrelin administration stimulates GH secretion but also causes weight gain by increasing food intake and reduction in fat utilization and anti-Ghrelin IgG administration suppressed feeding.
Product Categories/Family for anti-GHRL antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
appetite-regulating hormone
NCBI Official Symbol
GHRL
NCBI Protein Information
appetite-regulating hormone; preproghrelin; ghrelin/obestatin preprohormone
Protein Family
Research Articles on GHRL
Similar Products
Product Notes
The GHRL (Catalog #AAA613690) is an Antibody produced from Chicken and is intended for research purposes only. The product is available for immediate purchase. The Ghrelin reacts with Bovine, Canine, Human, Mouse, Porcine, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Ghrelin can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA, Western Blot, Antibody Blocking. Dilution: Western Blot: 1-10ug/ml using ECL. Full length ghrelin is ~3.5kD. ELISA: 1:100,000 using 50-100ng control peptide/well. Antibody Blocking: Use 5-10ug of control peptide per 1ug antibody. Researchers should empirically determine the suitability of the GHRL for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ghrelin, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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