Western Blot (WB)
(Western blot analysis of anti-TBB1 Antibody in mouse brain tissue, Y79, CEM and 293 lysates (35ug/lane). TBB1 (arrow) was detected using the purified Mab. (1ug/ml))
Mouse anti-Human, Mouse TBB1 Monoclonal Antibody | anti-TBB1 antibody
TBB1 (Tubulin beta-1 chain, TUBB1) (PE)
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Purified by Protein G Affinity Chromatography
Synonyms
TBB1; Monoclonal Antibody; TBB1 (Tubulin beta-1 chain; TUBB1) (PE); anti-TBB1 antibody
Host
Mouse
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10i79
Specificity
Recognizes human TBB1. Species Crossreactivity: mouse.
Purity/Purification
Purified by Protein G Affinity Chromatography
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Applicable Applications for anti-TBB1 antibody
FLISA, Western Blot (WB)
Application Notes
FLISA: 1:1,000
WB: 1:100-1:500
Applications are based on unconjugated antibody.
WB: 1:100-1:500
Applications are based on unconjugated antibody.
Immunogen
Recombinant protein human TBB1.
Conjugate
PE
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-TBB1 Antibody in mouse brain tissue, Y79, CEM and 293 lysates (35ug/lane). TBB1 (arrow) was detected using the purified Mab. (1ug/ml))
Western Blot (WB)
(Western blot analysis of anti-TBB1 Antibody in mouse brain tissue, Y79, CEM and 293 lysates (35ug/lane). TBB1 (arrow) was detected using the purified Mab. (1ug/ml))
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain reacted with TBB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of TBB1 Antibody with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of TBB1 Antibody with HepG2 cell followed by Alexa Fluor® 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).)
Product Categories/Family for anti-TBB1 antibody
References
1. Ragab, A., et al., J. Biol. Chem. 278(42):40923-40932 (2003). 2. Sun, J.P., et al., J. Biol. Chem. 278(14):12406-12414 (2003). 3. Boute, N., et al., EMBO Rep. 4(3):313-319 (2003). 4. Li, S., et al., Arch. Biochem.Biophys. 410(2):269-279 (2003). 5. Yigzaw, Y., et al., J. Biol. Chem. 278(1):284-288 (2003).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
50,327 Da
NCBI Official Full Name
tubulin beta-1 chain
NCBI Official Synonym Full Names
tubulin, beta 1 class VI
NCBI Official Symbol
TUBB1
NCBI Protein Information
tubulin beta-1 chain; beta tubulin 1, class VI; class VI beta-tubulin
UniProt Protein Name
Tubulin beta-1 chain
UniProt Gene Name
TUBB1
UniProt Entry Name
TBB1_HUMAN
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Product Notes
The TBB1 tubb1 (Catalog #AAA6121763) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The TBB1 (Tubulin beta-1 chain, TUBB1) (PE) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TBB1 can be used in a range of immunoassay formats including, but not limited to, FLISA, Western Blot (WB). FLISA: 1:1,000 WB: 1:100-1:500 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the TBB1 tubb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TBB1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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