Mouse anti-Human Actin, beta Monoclonal Antibody | anti-ACTB antibody

Actin, beta (Actin, cytoplasmic 1, Beta-actin, ACTB) (PE)

Cat. Num. AAA6121613

• Gene Names: ACTB; BRWS1; PS1TP5BP1
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Actin; beta; Monoclonal Antibody; beta (Actin; cytoplasmic 1; Beta-actin; ACTB) (PE); anti-ACTB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 10i27
• Specificity: Recognizes human Actin, beta.
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ACTB antibody: FLISA, Western Blot (WB)
• Application Notes: FLISA: 1:1,000; WB: 1:2,000-1:10,000; Applications are based on unconjugated antibody.
• Immunogen: ACTB recombinant protein.
• Conjugate: PE
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of anti-Beta-actin Monoclonal Antibody in HL-60 cell line lysates (36ug/lane). Beta-actin (arrow) was detected using the purified Mab.)

Immunohistochemistry (IHC)

(Beta-actin Monoclonal Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Beta-actin Monoclonal Antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

Product Categories/Family for anti-ACTB antibody

Antibodies; Actin Related Proteins

References

1. Villebeck,L., Biochemistry 46 (44), 12639-12647 (2007). 2. Avizienyte,E., Exp. Cell Res. 313 (15), 3175-3188 (2007). 3. Bouldin,A.A., Muscle Nerve 35 (2), 254-258 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4501885
• NCBI GeneID: 60
• NCBI Accession #: NP_001092
• NCBI GenBank Nucleotide #: NM_001101.4
• UniProt Accession #: P60709; P02570; P70514; P99021; Q11211; Q64316; Q75MN2; Q96B34; Q96HG5
• Molecular Weight: 41,737 Da
• NCBI Official Full Name: actin, cytoplasmic 1
• NCBI Official Synonym Full Names: actin beta
• NCBI Official Symbol: ACTB
• NCBI Official Synonym Symbols: BRWS1; PS1TP5BP1
• NCBI Protein Information: actin, cytoplasmic 1
• UniProt Protein Name: Actin, cytoplasmic 1
• Protein Family: Actin
• UniProt Gene Name: ACTB
• UniProt Entry Name: ACTB_HUMAN

NCBI Description

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins that are ubiquitously expressed. Mutations in this gene cause Baraitser-Winter syndrome 1, which is characterized by intellectual disability with a distinctive facial appearance in human patients. Numerous pseudogenes of this gene have been identified throughout the human genome. [provided by RefSeq, Aug 2017]