Mouse anti-Human, Mouse Myoglobin Monoclonal Antibody | anti-MB antibody

Myoglobin (MB, Myoglobin) (Azide free) (HRP)

Cat. Num. AAA6121551

• Gene Names: MB; PVALB
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography
• Synonyms: Myoglobin; Monoclonal Antibody; Myoglobin (MB; Myoglobin) (Azide free) (HRP); MB; anti-MB antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 444CT19.2.1
• Specificity: Recognizes human Myoglobin. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).

• Applicable Applications for anti-MB antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 103-130 of human Myoglobin, UniProt Accession #P02144. Species sequence homology: monkey, porcine
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart and skeletal muscle tissue lysates (35ug/lane) using. This demonstrates that detected the Myoglobin protein (arrow).)

Related Product Information for anti-MB antibody

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported.

Product Categories/Family for anti-MB antibody

Antibodies; Transcription Factors

References

1. Esquerra, R.M., et al. Phys Chem Chem Phys 12(35):10270-10278(2010) 2. Endeward, V., et al. Cardiovasc. Res. 87(1):22-29(2010) 3. Balobanov, V.A., et al. Mol. Biol. (Mosk.) 44(4):708-717(2010) 4. Kristiansen, G., et al. Br. J. Cancer 102(12):1736-1745(2010) 5. Rayner, B.S., et al. Biochem. J. 423(2):169-177(2009)

NCBI and Uniprot Product Information

• NCBI GI #: 4885477
• NCBI GeneID: 4151
• NCBI Accession #: NP_005359.1
• NCBI GenBank Nucleotide #: NM_005368.2
• UniProt Accession #: P02144; Q52H51; Q5THY7
• Molecular Weight: 17,184 Da
• NCBI Official Full Name: myoglobin
• NCBI Official Synonym Full Names: myoglobin
• NCBI Official Symbol: MB
• NCBI Official Synonym Symbols: PVALB
• NCBI Protein Information: myoglobin
• UniProt Protein Name: Myoglobin
• Protein Family: Myoglobin
• UniProt Gene Name: MB
• UniProt Entry Name: MYG_HUMAN

NCBI Description

This gene encodes a member of the globin superfamily and is expressed in skeletal and cardiac muscles. The encoded protein is a haemoprotein contributing to intracellular oxygen storage and transcellular facilitated diffusion of oxygen. At least three alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

MB: Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles. Belongs to the globin family.

Protein type: Carrier

Chromosomal Location of Human Ortholog: 22q13.1

Molecular Function: iron ion binding; heme binding; oxygen binding; oxygen transporter activity

Biological Process: slow-twitch skeletal muscle fiber contraction; response to hydrogen peroxide; oxygen transport; heart development; response to hormone stimulus; response to hypoxia; brown fat cell differentiation; enucleate erythrocyte differentiation