Mouse anti-Human Heparan Sulfate Monoclonal Antibody

Heparan Sulfate (10E4 epitope) (Biotin)

Cat. Num. AAA6120738

• Reactivity: Human
• Applications: ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by Tangential Ultrafiltration.
• Synonyms: Heparan Sulfate; Monoclonal Antibody; Heparan Sulfate (10E4 epitope) (Biotin); anti-Heparan Sulfate antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM,k
• Clone Number: 8.S.087
• Specificity: Recognizes an epitope present in many types of human heparan sulfate. The epitope includes N-sulfated glucosamine residues that are critical for the reactivity of the antibody. Does not react with hyaluronan, chondroitin sulfate, dermatan sulfate, keratan sulfate or DNA. Reactivity with most heparan sulfates is nearly completely abolished after treatment of the glycosaminoglycan with bacterial heparitinase (Flavobacterium heparinum, EC 4.2.2.8).
• Purity/Purification: Purified by Tangential Ultrafiltration.
• Form/Format: Supplied as a liquid in PBS. Labeled with Biotin.
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-Heparan Sulfate antibody: ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Liposome-incorporated membrane heparan sulfate proteoglycan from human fetal lung fibroblasts.
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-Heparan Sulfate antibody

Heparan sulfate, otherwise known as heparitin sulfate or heparin monosulfate, is a generic term describing polysaccharides which are linear and consist of N-acetylated [->4)alpha-D-GlcNpAc-(1->4) ß-D-GlcAp(1->] and N-sulfated disaccharides [->4)alpha-D-GlcNpS- (1->4)-ß-D-GlcAp or alpha-L-IdoAp(1->] that are arranged mainly in a segregated manner. The sulfate-rich fractions in heparan sulfate are heparin-like, though they rarely possess the sulfate density found in heparin. Approximately 25% of the total polymer is initially formed by alternating arrangements of the two disaccharide units, ->4)alpha-D-GlcNpS(1->4)UAp(1->4) alpha-D-GlcNpAc(1->4)UAp (1->4)-alpha-D-GlcAp(1->. The polymer is formed as a repeating ->4)-alpha-D-GlcNpAc (1->4)-ß-D-GlcAp(1-> disaccharide sequence that is attached to a serine residue of a core protein through a tetrasaccharide, glucuronosyl->galactosyl->galactosyl->xylosyl, linkage region. It then undergoes partial N-deacetylation followed by N-sulfation of the newly exposed amino groups, partial C-5 epimerization of D-GlcAp to L-IdoAp and O-sulfation. O-sulfates are always found in proximity to N-sulfates which enhances the clustering of the sulfate residues and the heterogeneity in chemical composition and charge density of heparan sulfate.

Product Categories/Family for anti-Heparan Sulfate antibody

Antibodies; Carbohydrates; Glycoproteins

References

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