Transferrin Recombinant Protein | TF recombinant protein

Human Transferrin protein

Cat. Num. AAA9719249

• Gene Names: TF; TFQTL1; PRO1557; PRO2086
• Purity: >=95 % as determined by SDS-PAGE
• Synonyms: Transferrin; Human Transferrin protein; PRO2086 Protein; TFQTL1 Protein; HEL-S-71p Protein; Human; PRO1558 Protein; Transferrin Protein; Siderophilin; partially saturated; TF recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >=95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4.

• Tag: TF
• Protein Construction: The recombinant Human Transferrin protein consists of 690 amino acids and has a predicted molecular mass of 76.6kDa. The apparent molecular mass of the Human Transferrin protein is approximately 70-100kDa in SDS-PAGE under reducing conditions.; Amino acid sequence derived from Human Transferrin protein isoform (P02787, Met 1-Pro 698) with a polyhistidine tag at the C-terminus was expressed.
• Usage Notes: Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human Transferrin protein using the buffer we provided not less than 100 ug/mL, which can then be further diluted to other aqueous solutions.
• Preparation and Storage: Lyophilized Human Transferrin proteinshould be stored desiccated below -20 degree C. Upon reconstitution, the protein should be Store at 4 degree C between 2-7 days and for future use below -20 degree C. For long term storage it is recommended to add a carrier protein (0.128% HSA or BSA). Please prevent freeze-thaw cycles.; Shipping||The product is shipped at ambient temperature.

SDS-Page

(Fig.SDS-PAGE analysis of Human Transferrin protein.)

Related Product Information for TF recombinant protein

Description: Human Transferrin protein, expressed in HEK293 Cells
Background: Transferrin (Tf),is an iron transporter, which is mainly biosynthesized in the liver, and it can also be biosynthesized in the brain; i.e., by oligodendrocytes and the choroid plexus, cerebrospinal fluid (CSF) producing tissue. The CSF contains two Tf isoforms, brain-type Tf and serum-type Tf, which differ in their glycan structures. Brain-type Tf is uniquely glycolsylated with biantennary asialo- and agalacto-complex type N-glycans that carry bisecting beta1,4-GlcNAc and core alpha1,6-Fuc.The glycans of serum-type Tf in the CSF are similar to those of Tf in serum. Biochemical analyses reveal that the apparent molecular size of brain-type Tf is smaller than that of serum-type Tf, and that hydrophobic patches are exposed on brain-type Tf as demonstrated by hydrophobic probe binding studies. Research indicates that brain-type Tf levels is decreased in idiopathic normal pressure hydrocephalus, in which CSF production is suspected to decrease, while brain-type Tf increased in spontaneous intracranial hypotension, in which CSF production is suspected to increase. These results suggest that brain-type Tf could be a biomarker of altered CSF production.

Product Categories/Family for TF recombinant protein

Cytokines; Cell Culture; Cytology

NCBI and Uniprot Product Information

• NCBI GI #: 339453
• NCBI GeneID: 7018
• UniProt Accession #: P02787; O43890; Q1HBA5; Q9NQB8; Q9UHV0
• Molecular Weight: 77,064 Da
• NCBI Official Full Name: transferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: TF
• NCBI Official Synonym Symbols: TFQTL1; PRO1557; PRO2086
• NCBI Protein Information: serotransferrin; siderophilin; beta-1 metal-binding globulin
• UniProt Protein Name: Serotransferrin
• Protein Family: Transferrin
• UniProt Gene Name: TF
• UniProt Synonym Gene Names: Transferrin
• UniProt Entry Name: TRFE_HUMAN

NCBI Description

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: extracellular space; cell surface; early endosome; cytoplasmic membrane-bound vesicle; extracellular region; coated pit; recycling endosome; endocytic vesicle; perinuclear region of cytoplasm; apical plasma membrane; late endosome; basal plasma membrane; endosome membrane; vesicle

Molecular Function: protein binding; ferric iron binding; ubiquitin protein ligase binding

Biological Process: platelet activation; retinal homeostasis; platelet degranulation; cellular iron ion homeostasis; transferrin transport; blood coagulation; transmembrane transport

Disease: Atransferrinemia