Western Blot (WB)
(Immunoprecipitation of Akt from PDGF (100ng/ml for 11 minutes) treated and untreated NIH/3T3 cell lysates, using MBS6120701 (left) and control Akt Antibody # (right). Western blot detection was performed using.)
Mouse AKT1 Monoclonal Antibody | anti-AKT1 antibody
AKT1 (v-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) (BSA, Azide & Glycerol Free)
Reactivity
Hamster, Human, Mouse, Rat
Applications
ELISA, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Purity
Ascites
Synonyms
AKT1; Monoclonal Antibody; AKT1 (v-akt Murine Thymoma Viral Oncogene Homolog 1; C-AKT; MGC99656; PKB; PRKBA; Protein kinase B; RAC; RAC-ALPHA; RAC-alpha serine/threonine-protein kinase; RAC-PK-alpha) (BSA; Azide & Glycerol Free); anti-AKT1 antibody
Host
Mouse
Reactivity
Hamster, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3A20
Specificity
Recognizes endogenous levels of human Akt at ~60kD. Does not crossreact with other related proteins. Species Crossreactivity: mouse, rat, hamster.
Purity/Purification
Ascites
Form/Format
Supplied as liquid in PBS, pH 7.2. No preservative added. No stabilizing proteins added. Available with azide, BSA and glycerol.
Applicable Applications for anti-AKT1 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
Application Notes
IP: 1:100
FC/FACS: 1:50
FC/FACS: 1:50
Immunogen
Recombinant protein corresponding to aa140-480 of human Akt1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Immunoprecipitation of Akt from PDGF (100ng/ml for 11 minutes) treated and untreated NIH/3T3 cell lysates, using MBS6120701 (left) and control Akt Antibody # (right). Western blot detection was performed using.)
Western Blot (WB)
(Immunoprecipitation of Akt from PDGF (100ng/ml for 11 minutes) treated and untreated NIH/3T3 cell lysates, using MBS6120701 (left) and control Akt Antibody # (right). Western blot detection was performed using.)
Testing Data
(MBS6120701 staining of untreated (blue) or LY-treated (green) jurkat cells compared to a nonspecific negative control antibody (red).)
Testing Data
(MBS6120701 staining of untreated (blue) or LY-treated (green) jurkat cells compared to a nonspecific negative control antibody (red).)
Immunofluorescence (IF)
(Confocal immunofluorescence images of C2C12 cells showing nuclear and cytoplasmic localization with MBS6120701 (left, red) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin.)
Immunofluorescence (IF)
(Confocal immunofluorescence images of C2C12 cells showing nuclear and cytoplasmic localization with MBS6120701 (left, red) compared to an isotype control (right). Actin filaments have been labeled with fluorescein phalloidin.)
Product Categories/Family for anti-AKT1 antibody
References
1. Franke, T.F. (1997) Cell 88, 435-437. 2. Burgering, B.T. and Coffer, P.J. (1995) Nature 376, 599-602. 3. Franke, T. F. et al. (1995) Cell 81, 727-736. 4. Alessi, D. R. et al. (1996) EMBO J. 15, 6541-6551. 5. Sarbassov, D.D. et al. (2005) Science 307, 1098-101. 6. Jacinto, E. et al. (1998) Cell 96, 857-68. 7. Cardone, M. H. et al. (1998) Science 282, 1318-1321. 8. Brunet, A. et al. (1999) Cell 96, 857-868. 9. Zimmermann, S. et al. (1999) Science 286, 1741-4. 10. Cantley, L. C. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 4240-4245. 11. Vlahos, C. et al. (1994) J. Biol. Chem. 269, 5241-5248.
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Product Notes
The AKT1 (Catalog #AAA6120701) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The AKT1 (v-akt Murine Thymoma Viral Oncogene Homolog 1, C-AKT, MGC99656, PKB, PRKBA, Protein kinase B, RAC, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, RAC-PK-alpha) (BSA, Azide & Glycerol Free) reacts with Hamster, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's AKT1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP). IP: 1:100 FC/FACS: 1:50. Researchers should empirically determine the suitability of the AKT1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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