Rabbit anti-Mouse EEA1 Polyclonal Antibody | anti-EEA1 antibody
EEA1 (Early Endosomal Antigen 1, Early Endosome Associated Protein, Endosome Associated Protein p162, ZFYVE2, Zinc Finger FYVE Domain Containing Protein 2)
Gene Names
EEA1; MST105; ZFYVE2; MSTP105
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
EEA1; Polyclonal Antibody; EEA1 (Early Endosomal Antigen 1; Early Endosome Associated Protein; Endosome Associated Protein p162; ZFYVE2; Zinc Finger FYVE Domain Containing Protein 2); Anti -EEA1 (Early Endosomal Antigen 1; anti-EEA1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
Recognizes mouse EEA1. Detects a band of ~160kD by Western Blot.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Applicable Applications for anti-EEA1 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Immunogen
Synthetic peptide corresponding to aa 1391-1410 (T1391PSSKKPVR VCDACFNDLQG1410) of human EEA1 (early endosomal antigen 1).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-EEA1 antibody
Early endosomal antigen 1 (EEA1) is a 162kD membrane bound protein component specific to the early endosome and is essential for fusion between early endocytic vesicles. Early endosomes are cytoplasmic compartments which fuse with endocytic vesicles for redistribution of extracellular compounds to alternate destinations. Zinc-finger-like domains, reminiscent of those found in nucleic acid binding proteins, are located in the amino and carboxyl-terminal domains of EEA1. The carboxyl-terminal zinc-finger-like-domain is conserved in several other non-nuclear proteins, some of which are also involved in intracellular protein trafficking. In addition, this domain is an authentic zinc-binding domain and is critical to the intracellular localization of EEA1. Membrane association of EEA1 has been shown dependent on phosphatidyl 3-kinase activity, inhibitors of which cause EEA1 to dissociate from early endosomes.
Product Categories/Family for anti-EEA1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
162,466 Da
NCBI Official Full Name
early endosome antigen 1
NCBI Official Synonym Full Names
early endosome antigen 1
NCBI Official Symbol
EEA1
NCBI Official Synonym Symbols
MST105; ZFYVE2; MSTP105
NCBI Protein Information
early endosome antigen 1; early endosome antigen 1, 162kD; endosome-associated protein p162; early endosome-associated protein; zinc finger FYVE domain-containing protein 2
UniProt Protein Name
Early endosome antigen 1
Protein Family
UniProt Gene Name
EEA1
UniProt Synonym Gene Names
ZFYVE2
UniProt Entry Name
EEA1_HUMAN
Uniprot Description
EEA1: Binds phospholipid vesicles containing phosphatidylinositol 3-phosphate and participates in endosomal trafficking. Homodimer. Binds STX6. Binds RAB5A, RAB5B, RAB5C and RAB22A that have been activated by GTP-binding. Interacts with ERBB2.
Protein type: C2H2-type zinc finger protein; Vesicle
Chromosomal Location of Human Ortholog: 12q22
Cellular Component: extrinsic to plasma membrane; recycling endosome; membrane; early endosome membrane; early endosome; cytoplasm; cytoplasmic vesicle; cytosol; serine-pyruvate aminotransferase, type 2B complex
Molecular Function: calmodulin binding; protein binding; protein homodimerization activity; zinc ion binding; GTP-dependent protein binding; phosphatidylinositol binding
Biological Process: vesicle fusion; early endosome to late endosome transport; synaptic vesicle to endosome fusion; endocytosis
Research Articles on EEA1
Similar Products
Product Notes
The EEA1 eea1 (Catalog #AAA612067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The EEA1 (Early Endosomal Antigen 1, Early Endosome Associated Protein, Endosome Associated Protein p162, ZFYVE2, Zinc Finger FYVE Domain Containing Protein 2) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EEA1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the EEA1 eea1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EEA1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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