Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS611889.)
Rabbit anti-Human Smad Ubiquitination Regulatory Factor-1 Polyclonal Antibody | anti-SMURF1 antibody
Smad Ubiquitination Regulatory Factor-1 (Smurf1)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedAffinity Purified.
Synonyms
Smad Ubiquitination Regulatory Factor-1; Polyclonal Antibody; Smad Ubiquitination Regulatory Factor-1 (Smurf1); Anti -Smad Ubiquitination Regulatory Factor-1 (Smurf1); anti-SMURF1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Specificity
Detects endogenous levels of total Smurf1 protein.
Purity/Purification
Affinity Purified
Affinity Purified.
Affinity Purified.
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA and 50% glycerol.
Applicable Applications for anti-SMURF1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western blot 1:1000
Dilution: Western blot 1:1000
Immunogen
Synthetic peptide (KLH-coupled) corresponding to a central region within human Smurf1.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS611889.)
Western Blot (WB)
(Western blot analysis of extracts from various cell lines using MBS611889.)
Related Product Information for anti-SMURF1 antibody
Bone morphogenetic proteins (BMPs) constitute a large family of signaling molecules that regulate a wide range of critical processes including morphogenesis, cell-fate determination, proliferation, differentiation and apoptosis (1,2). BMP receptors are members of the TGF- beta family of Ser/Thr kinase receptors. Ligand binding induces multimerization, autophosphorylation and activation of these receptors (3-5). Subsequently, they phosphorylate Smad1 at Ser463 and Ser465 in the carboxy-terminal motif SSXS, as well as Smad5 and Smad8 at their corresponding sites. These phosphorylated Smads dimerize with the collaborating Smad4 and translocate to the nucleus, where the transcription of target genes is stimulated (5). Smurf1, a member of the HECT family of E3 ubiquitin ligases, seletively interacts with Smads of the BMP pathway, leading to their ubiquitination and degradation (6). In addition, Smurf1 has been shown to interact with the inhibitor Smad, Smad7, the bone-specific transcription factor Runx2/Cbfa1, RhoA and MEKK2 (7-10). In vivo, Smurf1 negatively regulates osteoblast differentiation and bone formation (10,11). A related protein, Smurf2, acts more promiscuously, interacting with both BMP and TGF-b Smad proteins (12).
Product Categories/Family for anti-SMURF1 antibody
NCBI and Uniprot Product Information
Similar Products
Product Notes
The SMURF1 (Catalog #AAA611889) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Smad Ubiquitination Regulatory Factor-1 (Smurf1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Smad Ubiquitination Regulatory Factor-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western blot 1:1000. Researchers should empirically determine the suitability of the SMURF1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smad Ubiquitination Regulatory Factor-1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
