Rabbit Musashi-1 RNA Binding Protein Polyclonal Antibody | anti-MSI1 antibody

Musashi-1 RNA Binding Protein (Musashi 1, Musashi1, Msi1, Msi-1, Musashi Homolog 1, RNA Binding Protein Musashi Homolog 1)

Cat. Num. AAA611647

• Reactivity: Human, Mouse, Rat; ; Species Crossreactivity: rat and mouse.
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Immunoaffinity chromatography.
• Synonyms: Musashi-1 RNA Binding Protein; Polyclonal Antibody; Musashi-1 RNA Binding Protein (Musashi 1; Musashi1; Msi1; Msi-1; Musashi Homolog 1; RNA Binding Protein Musashi Homolog 1); Anti -Musashi-1 RNA Binding Protein (Musashi 1; anti-MSI1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; ; Species Crossreactivity: rat and mouse.
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Musashi-1 RNA binding protein.
• Purity/Purification: Affinity Purified; Purified by Immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-MSI1 antibody: Western Blot (WB), Immunohistochemistry (IHC-F/P), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in:; ; Western Blot: 1:500; Immunocytochemistry (Frozen/Paraffin): 1:10-1:2000
• Immunogen: Synthetic peptide corresponding to aa5-21, APQPGLASPDSPHDPCK of human Musashi-1 binding protein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Image of staining with MBS611647 (1:100) shows Musashi (green) staining on neural rosettes (human). Nuclei are counterstained blue (DAPI). Cells were fixed with 4% PFA. No antigen retrieval was done.)

Immunohistochemistry (IHC)

(Musashi-positive cells stained with MBS611647 (1:150) in the ventricular zone of E14.5 mouse medulla. Image taken at 200x magnification. The sections were 4% PFA fixed, paraffin-embedded and cut at 5 micron)

Western Blot (WB)

(Western Blot analysis of Musashi-1 with MBS611647 (1:500). 10ug of rat neural stem cell lysate (lane 1) and normal human brain lysate (lane 2))

Related Product Information for anti-MSI1 antibody

Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Musashi-1 (Msi-1) has been shown to increase the accumulation of tau isoforms in intracellular inclusions in dementia. It may play a role in the pathogenesis of various neurodegenerative disorders.
Musashi1 has also been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in cancer development. Msi-1 also appears to play be a vital role in the development of several types of carcinoma such as human heptoma, and may be a useful molecular marker for tumor detection.

Product Categories/Family for anti-MSI1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 3786320
• NCBI GeneID: 4440
• UniProt Accession #: O43347; Q96PU0; Q96PU1; Q96PU2; Q96PU3
• Molecular Weight: 39,125 Da
• NCBI Official Full Name: Musashi
• NCBI Official Synonym Full Names: musashi RNA-binding protein 1
• NCBI Official Symbol: MSI1
• NCBI Protein Information: RNA-binding protein Musashi homolog 1; musashi1; musashi-1
• UniProt Protein Name: RNA-binding protein Musashi homolog 1
• Protein Family: RNA-binding protein
• UniProt Gene Name: MSI1
• UniProt Synonym Gene Names: Musashi-1
• UniProt Entry Name: MSI1H_HUMAN

NCBI Description

This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13. [provided by RefSeq, Jul 2008]

Uniprot Description

Musashi-1: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'- GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'- [GA]U(1-3)AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system. Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. Belongs to the Musashi family.

Protein type: RNA-binding

Chromosomal Location of Human Ortholog: 12q24

Cellular Component: polysome; cytoplasm; nucleus

Molecular Function: RNA binding; nucleotide binding; poly(U) binding

Biological Process: nervous system development; epithelial cell differentiation; response to hormone stimulus