Western Blot (WB)
(Western blot analysis of extracts of various cell lines using MBS611455.)
Rabbit anti-Human XLF Polyclonal Antibody
XLF (XRCC4-like Factor, Cernunnos, FLJ12610, Nonhomologous End Joining Factor 1, Non-homologous End-joining Factor 1, NHEJ 1, NHEJ1, Protein Cernunnos)
Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Purity
Purified by peptide affinity chromatography.
Synonyms
XLF; Polyclonal Antibody; XLF (XRCC4-like Factor; Cernunnos; FLJ12610; Nonhomologous End Joining Factor 1; Non-homologous End-joining Factor 1; NHEJ 1; NHEJ1; Protein Cernunnos); Anti -XLF (XRCC4-like Factor; anti-XLF antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous levels of total XLF protein.
Purity/Purification
Purified by peptide affinity chromatography.
Form/Format
Supplied as a liquid in 0 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Applicable Applications for anti-XLF antibody
Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in Western Blot and Immunoprecipitation.
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Dilution: Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunogen
Synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy terminus of human XLF.
Preparation and Storage
For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using MBS611455.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines using MBS611455.)
Related Product Information for anti-XLF antibody
Double stranded DNA breaks (DSB's) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. The mechanism by which cells deal with DSB's is known as NHEJ (non-homologous end-joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1). XLF, also known as Cernunnos, was originally discovered as a mutated protein from cells of individuals who displayed features of growth retardation, microcephaly, and immunodeficiency (2). These cells were sensitive to ionizing radiation and defective in V(D)J recombination. Exogenous expression of wild type XLF corrected these deficiencies (3), indicating that XLF is a critical component of the NHEJ response. XLF physically interacts with and may stimulate the ligase activity of XRCC4 (3).
Product Categories/Family for anti-XLF antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
XLF family protein
Similar Products
Product Notes
The XLF (Catalog #AAA611455) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The XLF (XRCC4-like Factor, Cernunnos, FLJ12610, Nonhomologous End Joining Factor 1, Non-homologous End-joining Factor 1, NHEJ 1, NHEJ1, Protein Cernunnos) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's XLF can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Immunoprecipitation: 1:100. Researchers should empirically determine the suitability of the XLF for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "XLF, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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