Rabbit Heat Shock Protein 110 Polyclonal Antibody | anti-Hsph1 antibody

Heat Shock Protein 110 (HSP110)

Cat. Num. AAA611330

• Gene Names: Hsph1; 105kDa; Hsp105; Hsp110; hsp-E7I; AI790491
• Reactivity: Bovine, Hamster, Human, Monkey, Mouse, Rat, Sheep, Yeast
• Applications: Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography
• Synonyms: Heat Shock Protein 110; Polyclonal Antibody; Heat Shock Protein 110 (HSP110); Anti -Heat Shock Protein 110 (HSP110); anti-Hsph1 antibody

• Host: Rabbit
• Reactivity: Bovine, Hamster, Human, Monkey, Mouse, Rat, Sheep, Yeast
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes a ~110kD protein, corresponding to the apparent molecular mass of human Hsp110. Species Crossreactivity: monkey, hamster, rat, mouse, bovine, sheep and yeast cell lines.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide, 50% glycerol.

• Applicable Applications for anti-Hsph1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation and Western Blot.; Dilution: Western Blot: 1ug/ml (colorimetric); Optimal dilution to be determined by researcher.
• Immunogen: Synthetic peptide derived from the sequence of hamster Hsp 110. Sequence identical to human and mouse.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Hsph1 antibody

Hsp 110 (110kD) belongs to a family of large stress proteins referred to as the Hsp110/SSE family (1). The proteins in this family are the most distantly known relatives of the well studied Hsp70 family. Members of this group are significantly larger in size and contain sequences not present in members of the Hsp70 family. The mammalian protein has recently been cloned (1) and found to share 30-33% amino acid identity with members of the Hsp70 family, most of which occurs in the conserved ATP-binding domain of these molecules. Presently, all identified members of the Hsp70-like sequences can be categorized as being members of either one of these two groups; i.e. the DnaK (i.e. Hsp70) family or the Hsp110/SSE family (1). While most stress protein families (20kD, 60kD, 70kD, and 90kD) have been studied extensively, the Hsp110 species has been virtually ignored. Reports to date indicate that Hsp110 is a normal constituent of mammalian cells, it is induced by heat shock and its induction strongly correlates with the expression of thermotolerance (2). Furthermore, Hsp110 has been shown to be associated with the nucleolus (3) and Western blot analysis of several murine tissues indicates that Hsp110 is constitutively expressed at varying levels in all mouse tissues and is highly expressed in brain (1).

Product Categories/Family for anti-Hsph1 antibody

Antibodies; Abs to Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 114145505
• NCBI GeneID: 15505
• NCBI Accession #: NP_038587.2
• NCBI GenBank Nucleotide #: NM_013559.2
• UniProt Accession #: Q61699; Q3TNS2; Q3UIY8; Q62578; Q62579; Q6A0A5; Q8C430; Q8VCW6
• Molecular Weight: 96,407 Da
• NCBI Official Full Name: heat shock protein 105 kDa
• NCBI Official Synonym Full Names: heat shock 105kDa/110kDa protein 1
• NCBI Official Symbol: Hsph1
• NCBI Official Synonym Symbols: 105kDa; Hsp105; Hsp110; hsp-E7I; AI790491
• NCBI Protein Information: heat shock protein 105 kDa; HSP105 42 C-HSP; 42 degrees C-HSP; heat shock protein 110; heat shock 110 kDa protein; heat shock protein, 105 kDa; heat shock-related 100 kDa protein E7I
• UniProt Protein Name: Heat shock protein 105 kDa
• Protein Family: Heat shock protein
• UniProt Gene Name: Hsph1
• UniProt Synonym Gene Names: Hsp105; Hsp110; Kiaa0201; HSP-E7I
• UniProt Entry Name: HS105_MOUSE

Uniprot Description

HSP105: a molecular chaperone of the heat shock protein 70 family. Has ATPase activity. Expressed at especially high levels in mammalian brain and has been shown to suppress apoptosis in neuronal cells and to prevent the aggregation of proteins following heat shock. Known to interact with a variety of proteins including alpha-tubulin and the androgen receptor. Two alternatively spliced isoforms have been described.

Protein type: Chaperone; Heat shock protein

Cellular Component: nucleoplasm; microtubule; cytoplasm; extracellular region; cytosol; nucleus

Molecular Function: protein binding; nucleotide binding; alpha-tubulin binding; ATP binding

Biological Process: positive regulation of NK T cell activation; chaperone cofactor-dependent protein folding; positive regulation of MHC class I biosynthetic process; positive regulation of transcription from RNA polymerase II promoter; negative regulation of neuron apoptosis; response to unfolded protein