Immunohistochemistry (IHC)
(SMARCAL1(5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)
Rabbit anti-Human HepA-Related Protein Polyclonal Antibody | anti-SMARCAL1 antibody
HepA-Related Protein (HARP, SMARCAL1, EC 3.6.1.-, SIOD)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Immunohistochemistry: 5ug/ml
Western Blot: 1:500-1:1000
ELISA: 1:16,000-1:64,000
Immunohistochemistry (IHC)
(SMARCAL1(5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)
Immunohistochemistry (IHC)
(SMARCAL1(5ug/ml) was used in IHC to stain formalin-fixed, paraffin-embedded human brain, cortex, followed by biotinylated goat rabbit IgG secondary, alkaline phosphatase-streptavidin and chromogen.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein shows sequence similarity to the E. coli RNA polymerase-binding protein HepA. Mutations in this gene are a cause of Schimke immunoosseous dysplasia (SIOD), an autosomal recessive disorder with the diagnostic features of spondyloepiphyseal dysplasia, renal dysfunction, and T-cell immunodeficiency. [provided by RefSeq]
Uniprot Description
SMARCAL1: ATP-dependent annealing helicase that catalyzes the rewinding of the stably unwound DNA. Rewinds single-stranded DNA bubbles that are stably bound by replication protein A (RPA). Acts throughout the genome to reanneal stably unwound DNA, performing the opposite reaction of many enzymes, such as helicases and polymerases, that unwind DNA. Defects in SMARCAL1 are a cause of Schimke immuno-osseous dysplasia (SIOD). SIOD causes spondyloepiphyseal dysplasia, renal dysfunction and T-cell immunodeficiency. Approximately half of all patients also exhibit hyperthyroidism, while around half also exhibit episodal cerebral ischema. Belongs to the SNF2/RAD54 helicase family. SMARCAL1 subfamily.
Protein type: EC 3.6.1.-; EC 3.6.4.-; Helicase
Chromosomal Location of Human Ortholog: 2q35
Cellular Component: nucleoplasm; DNA replication factor A complex; nucleus
Molecular Function: DNA-dependent ATPase activity; protein binding; DNA binding; helicase activity; ATP binding
Biological Process: regulation of transcription from RNA polymerase II promoter; DNA strand renaturation; chromatin modification; DNA repair; response to DNA damage stimulus; DNA metabolic process; replication fork processing
Disease: Immunoosseous Dysplasia, Schimke Type
Research Articles on SMARCAL1
2. SMARCAL1 is a novel DNA damage-binding protein involved in replication fork stabilization.
3. ARP is recruited to stalled replication forks via its direct interaction with Replication protein A (RPA)
4. the interaction of HARP with RPA increases the concentration of annealing helicase activity in the vicinity of ssDNA regions to facilitate processes such as DNA repair
5. SMARCAL1 directly interacts with Replication protein A (RPA) and is recruited to sites of DNA damage in an RPA-dependent manner
6. SMARCAL1
7. Donor serum SMARCAL1 may serve as a specific, sensitive, and noninvasive predictive marker in the assessment of cardiac graft quality.
8. SMARCAL1 mutations: a cause of prepubertal idiopathic steroid-resistant nephrotic syndrome. (Case Report).
9. SMARCAL1 binds chromatin in vivo and that Schimke immuno-osseous dysplasia arises from impairment of diverse SMARCAL1 functions
10. study found that HARP is an ATP-dependent annealing helicase that rewinds single-stranded DNA bubbles that are stably bound by replication protein A
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Product Notes
The SMARCAL1 smarcal1 (Catalog #AAA611204) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The HepA-Related Protein (HARP, SMARCAL1, EC 3.6.1.-, SIOD) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HepA-Related Protein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, IImmunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry: 5ug/ml Western Blot: 1:500-1:1000 ELISA: 1:16,000-1:64,000. Researchers should empirically determine the suitability of the SMARCAL1 smarcal1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HepA-Related Protein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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