Rabbit anti-Rat Hydroxysteroid Dehydrogenase, 11-beta Type II Polyclonal Antibody
Hydroxysteroid Dehydrogenase, 11-beta Type II
Reactivity
Rat
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Hydroxysteroid Dehydrogenase; 11-beta Type II; Polyclonal Antibody; Anti -Hydroxysteroid Dehydrogenase; anti-Hydroxysteroid Dehydrogenase; 11-beta Type II antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Hydroxysteroid Dehydrogenase, 11-beta Type II. No significant sequence homology is seen with 11b-HSD1 or other proteins. Species crossreactivity: The rat peptide is not well conserved in mouse and human 11b-HSD2. We recommend the use for mouse and human 11b-HSD2, H9117-80 and H9117-50H respectively.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in 100mM Tris, pH 7.5, 0.2% BSA, 0.05% sodium azide, 40% glycerol.
Applicable Applications for anti-Hydroxysteroid Dehydrogenase, 11-beta Type II antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Dilution: Western Blot: 1-10ug/ml (ECL).
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.
Immunogen
A 19-aa synthetic peptide mapping near the C-terminus of rat 11b-HSD2 (1), coupled to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Hydroxysteroid Dehydrogenase, 11-beta Type II antibody
11-b-hydroxysteroid dehydrogenase (11b-HSD) is a microsomal short chain dehydrogenase/reductase (SDR) which catalyzes the inter-conversion of biologically active glucocorticoid (cortisol in human and corticosterone in rats and mice) and inactive glucocorticoid (cortisone and 11-dehydrocorticosterone). Two tissue specific isoforms (11beta-HSD1 and 11beta-HSD2) of 11b-HSD with two different functions regarding glucocorticoid availability, have been identified. The decreased 11b-hydroxy oxidation of cortisol results in Apparent Mineralocorticoid Excess (AME) disorder which is manifested by hypertension, hypokalemia, low plasma renin activity, and responsiveness to spironolactone. AME is principally a disorder of juveniles and children with this condition oxidize cortisol to cortisone poorly but carry out the reverse process unimpaired. AME arises from mutations in the 11beta-HSD2 gene. The glucocorticoids can also be produced locally by 11b-HSD1 and increased visceral accumulation of glucocorticoids results in visceral obesity, insulin resistant diabetes, hyperlipidemia and hyperphagia. 11betaHSD-2 (rat 400-aa, mouse 396-aa, human 405-aa) is a ~41kD glycosylated membrane-protein present in the endoplasmic reticulum (ER). The N-terminal and C-terminal (catalytic domain) of 11b-HSD2 are in the lumen and cytoplasm of ER, respectively. 11b-HSD2 irreversibly catalyzes the dehydrogenation of active 11b-hydroxycorticoids before they occupy mineralocorticoid receptors (MR). Thus confers aldosterone selectivity for inherently nonselective MR. The enzyme is expressed in a wide array of tissues, with highest level mineralocorticoid target cells such as the renal and outer medullary collecting ducts. In mouse, rat and Human, the over-all aa seq of 11b-HSD2 is >80% identical. In mouse, the over-all aa seq of 11b-HSD2 is >80% identical to that of 11b-HSD2.
Product Categories/Family for anti-Hydroxysteroid Dehydrogenase, 11-beta Type II antibody
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Product Notes
The Hydroxysteroid Dehydrogenase, 11-beta Type II (Catalog #AAA610911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hydroxysteroid Dehydrogenase, 11-beta Type II reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Hydroxysteroid Dehydrogenase, 11-beta Type II can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1-10ug/ml (ECL). ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Researchers should empirically determine the suitability of the Hydroxysteroid Dehydrogenase, 11-beta Type II for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hydroxysteroid Dehydrogenase, 11-beta Type II, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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