Rabbit anti-Human MCP3 Polyclonal Antibody | anti-CCL7 antibody
MCP3 (Macrophage/Monocyte Chemotactic Protein 3) (Biotin)
Gene Names
CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7; MGC138463; MGC138465
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Neutralization Assay
Purity
Affinity PurifiedPurified by immobilized antigen affinity chromatography.
Synonyms
MCP3; Polyclonal Antibody; MCP3 (Macrophage/Monocyte Chemotactic Protein 3) (Biotin); Anti -MCP3 (Macrophage/Monocyte Chemotactic Protein 3) (Biotin); anti-CCL7 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes MCP-3
Purity/Purification
Affinity Purified
Purified by immobilized antigen affinity chromatography.
Purified by immobilized antigen affinity chromatography.
Form/Format
A sterile filtered antibody solution (1mg/ml) was lyophilized from 0.5X PBS, pH 7.4. Conjugated to Biotin.
Applicable Applications for anti-CCL7 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Neutralization
Application Notes
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 1.75-3.0ug/ml of this antibody is required.
ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Immunohistochemistry: To be determined by the researcher.
Western Blot: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well.
Immunohistochemistry: To be determined by the researcher.
Western Blot: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions.
Immunogen
Highly purified (>98%) recombinant antigen.
Country of Origin
USA
Preparation and Storage
Lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 degree C. When reconstituted in sterile water to a concentration of 0.2mg/ml the antibody is stable for at least six weeks at 2-4 degree C.
Related Product Information for anti-CCL7 antibody
Human macrophage/monocyte chemotactic protein-3 (MCP-3) is a 8.5kD protein containing 76 amino acids residues. It plays an important role in the inflammatory response of blood monocytes, tissue macrophages, and eosinophils. The gene encoding this protein encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.
Product Categories/Family for anti-CCL7 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
11,200 Da
NCBI Official Full Name
monocyte chemoattractant protein 3
NCBI Official Synonym Full Names
chemokine (C-C motif) ligand 7
NCBI Official Symbol
CCL7
NCBI Official Synonym Symbols
FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7; MGC138463; MGC138465
NCBI Protein Information
C-C motif chemokine 7; OTTHUMP00000163801; small-inducible cytokine A7; monocyte chemotactic protein 3; monocyte chemoattractant protein 3; small inducible cytokine A7 (monocyte chemotactic protein 3)
UniProt Protein Name
C-C motif chemokine 7
Protein Family
UniProt Gene Name
CCL7
UniProt Synonym Gene Names
MCP3; SCYA6; SCYA7
UniProt Entry Name
CCL7_HUMAN
NCBI Description
This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq]
Uniprot Description
CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 17q11.2-q12
Cellular Component: extracellular space; extracellular region
Molecular Function: heparin binding; CCR1 chemokine receptor binding; chemokine activity; CCR2 chemokine receptor binding
Biological Process: cellular calcium ion homeostasis; monocyte chemotaxis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; immune response; cytoskeleton organization and biogenesis; inflammatory response; chemotaxis; signal transduction; positive regulation of cell migration
Research Articles on CCL7
1. The expression of MCP-3 in laryngeal squamous cell carcinoma is much higher than in normal tissue.
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Product Notes
The CCL7 ccl7 (Catalog #AAA610879) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MCP3 (Macrophage/Monocyte Chemotactic Protein 3) (Biotin) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's MCP3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Neutralization. Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity (50ng/ml), a concentration of 1.75-3.0ug/ml of this antibody is required. ELISA: Detection by direct ELISA (using 100ul/well antibody solution), requires a concentration of at least 0.5ug/ml of antibody. This antigen-affinity purified antibody, in conjunction with compatible secondary reagents, allows detection of 0.2-0.4ng/well. Immunohistochemistry: To be determined by the researcher. Western Blot: Use at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit is 1.5-3.0ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the CCL7 ccl7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MCP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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