Mouse anti-Human CCL7 Monoclonal Antibody | anti-CCL7 antibody

CCL7 (MCP3, SCYA6, SCYA7, C-C motif Chemokine 7, Monocyte Chemoattractant Protein 3, Monocyte Chemotactic Protein 3, NC28, Small-inducible Cytokine A7, MGC138463, MGC138465) (Biotin)

Cat. Num. AAA6141010

• Gene Names: CCL7; FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: CCL7; Monoclonal Antibody; CCL7 (MCP3; SCYA6; SCYA7; C-C motif Chemokine 7; Monocyte Chemoattractant Protein 3; Monocyte Chemotactic Protein 3; NC28; Small-inducible Cytokine A7; MGC138463; MGC138465) (Biotin); anti-CCL7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 4B5
• Specificity: Recognizes human CCL7.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-CCL7 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa24-100 from human CCL7 (NP_006264) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: QPVGINTSTTCCYRFINKKIPKQRLESYRRTTSSHCPREAVIFKTKLDKEICADPTQKWVQDFMKHLDKKTQTPKL*
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (34.47kD).)

Western Blot (WB)

(Western Blot analysis of CCL7 expression in transfected 293T cell line by CCL7 monoclonal antibody. Lane 1: CCL7 transfected lysate (11.2kD). Lane 2: Non-transfected lysate.)

Testing Data

(Detection limit for recombinant GST tagged CCL7 is ~1ng/ml as a capture antibody.)

Related Product Information for anti-CCL7 antibody

This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q.

Product Categories/Family for anti-CCL7 antibody

Antibodies; Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 13435402
• NCBI GeneID: 6354
• NCBI Accession #: NP_006264
• NCBI GenBank Nucleotide #: NM_006273
• UniProt Accession #: P80098; Q569J6
• Molecular Weight: 11,200 Da
• NCBI Official Full Name: C-C motif chemokine 7
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 7
• NCBI Official Symbol: CCL7
• NCBI Official Synonym Symbols: FIC; MARC; MCP3; NC28; MCP-3; SCYA6; SCYA7
• NCBI Protein Information: C-C motif chemokine 7
• UniProt Protein Name: C-C motif chemokine 7
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL7
• UniProt Synonym Gene Names: MCP3; SCYA6; SCYA7; MCP-3
• UniProt Entry Name: CCL7_HUMAN

NCBI Description

This gene encodes monocyte chemotactic protein 3, a secreted chemokine which attracts macrophages during inflammation and metastasis. It is a member of the C-C subfamily of chemokines which are characterized by having two adjacent cysteine residues. The protein is an in vivo substrate of matrix metalloproteinase 2, an enzyme which degrades components of the extracellular matrix. This gene is part of a cluster of C-C chemokine family members on chromosome 17q. [provided by RefSeq, Jul 2008]

Uniprot Description

CCL7: Chemotactic factor that attracts monocytes and eosinophils, but not neutrophils. Augments monocyte anti-tumor activity. Also induces the release of gelatinase B. This protein can bind heparin. Binds to CCR1, CCR2 and CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11.2-q12

Cellular Component: extracellular space; extracellular region

Molecular Function: heparin binding; CCR1 chemokine receptor binding; CCR2 chemokine receptor binding; chemokine activity

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; monocyte chemotaxis; cell-cell signaling; eosinophil chemotaxis; response to gamma radiation; cytoskeleton organization and biogenesis; immune response; inflammatory response; chemotaxis; signal transduction; positive regulation of cell migration