anti-Human CABP9K Polyclonal Antibody | anti-CABP9K antibody
Anti - CABP9K (CALB3, CABP1)
Gene Names
S100g; CABP1; Calb3; Cabp9k; AU042539; CaBP-D9K
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity purified over the control-peptide Sepharose
Synonyms
CABP9K; Polyclonal Antibody; Anti - CABP9K (CALB3; CABP1); CABP9K (CALB3; anti-CABP9K antibody
Host
Host: Rabbit
Source: Human
Source: Human
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
The peptide is 78% conserved in mouse, rat, bovine and porcine D9K. No significant sequence homology is seen with D28K or other CABPs. Antibody reactivity in various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity (see below).
Purity/Purification
Affinity purified over the control-peptide Sepharose
Form/Format
Affinity Purified
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide, 40% glycerol.
Sequence Length
79
Applicable Applications for anti-CABP9K antibody
ELISA, Western Blot
Application Notes
Suitable for use in ELISA, Western Blotting. Other applications not tested.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL.
ELISA: ~1:100,000 using 50-100ng control peptide/well.
Optimal dilutions to be determined by the researcher.
Immunogen
A 14-aa peptide sequence, within the N-terminal Ca-binding domain of Human D9K (1) was synthesized, coupled to KLH
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Product Categories/Family for anti-CABP9K antibody
References
• 1. Perret C et al (1988) Eur. J. Biochem. 172, 43-51; Howard A et al (1992) BBRC 185, 663-669; Jeung EB et al (1992) FEBS Lett. 307, 224-228; Jeung EB et al (1994) J. Mol. Biol. 235, 1231-1238; Colnot S et al (1998) JBC 273, 31939-31946;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
8,970 Da
NCBI Official Full Name
calcium binding protein D-9k
NCBI Official Synonym Full Names
S100 calcium binding protein G
NCBI Official Symbol
S100g
NCBI Official Synonym Symbols
CABP1; Calb3; Cabp9k; AU042539; CaBP-D9K
NCBI Protein Information
protein S100-G; CABP; calbindin D9k; OTTMUSP00000021020; calbindin-D9K major form; S100 calcium-binding protein G; cytidine 5'-triphosphate synthase 2; vitamin D-dependent calcium-binding protein, intestinal; calbindin 3, (vitamin D-dependent calcium binding protein)
UniProt Protein Name
Protein S100-G
UniProt Gene Name
S100g
UniProt Synonym Gene Names
Calb3; S100d
UniProt Entry Name
S100G_MOUSE
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Product Notes
The CABP9K s100g (Catalog #AAA610566) is an Antibody produced from Host: Rabbit Source: Human and is intended for research purposes only. The product is available for immediate purchase. The Anti - CABP9K (CALB3, CABP1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CABP9K can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot. Suitable for use in ELISA, Western Blotting. Other applications not tested. Dilution: Western Blot: 1:1000-1:5000 for neat serum and 1-10ug/ml for affinity pure using ECL. ELISA: ~1:100,000 using 50-100ng control peptide/well. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the CABP9K s100g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CABP9K, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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