Rabbit anti-Human, Mouse GRK1 Polyclonal Antibody | anti-GRK1 antibody

GRK1, CT (G-Protein-coupled Receptor Kinase 1, GPRK1, Rhodopsin Kinase, RHOK, RK) (Azide free) (HRP)

Cat. Num. AAA6478994

• Gene Names: GRK1; RK; RHOK; GPRK1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: GRK1; Polyclonal Antibody; CT (G-Protein-coupled Receptor Kinase 1; GPRK1; Rhodopsin Kinase; RHOK; RK) (Azide free) (HRP); EC=2.7.11.14; anti-GRK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human GRK1. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-GRK1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; ELISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human GRK1 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-GRK1 Pab is used in Western blot to detect GRK1 in HeLa cell lysate (Lane 1) and mouse spleen tissue lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GRK1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Product Categories/Family for anti-GRK1 antibody

Antibodies; G Protein Receptors

References

1. Zhao, X., et al., J. Biol. Chem. 273(9):5124-5131 (1998). 2. Zhao, X., et al., Vis. Neurosci. 14(2):225-232 (1997). 3. Yamamoto, S., et al., Nat. Genet. 15(2):175-178 (1997). 4. Khani, S.C., et al., Genomics 35(3):571-576 (1996). 5. Lorenz, W., et al., Proc. Natl. Acad. Sci. USA 88(19):8715-8719 (1991).

NCBI and Uniprot Product Information

• NCBI GI #: 4506529
• NCBI GeneID: 6011
• NCBI Accession #: NP_002920.1
• NCBI GenBank Nucleotide #: NM_002929.2
• UniProt Accession #: Q15835; Q53X14
• Molecular Weight: 63,526 Da
• NCBI Official Full Name: rhodopsin kinase
• NCBI Official Synonym Full Names: G protein-coupled receptor kinase 1
• NCBI Official Symbol: GRK1
• NCBI Official Synonym Symbols: RK; RHOK; GPRK1
• NCBI Protein Information: rhodopsin kinase
• UniProt Protein Name: Rhodopsin kinase
• UniProt Gene Name: GRK1
• UniProt Synonym Gene Names: RHOK; RK
• UniProt Entry Name: RK_HUMAN

NCBI Description

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]

Uniprot Description

GRK1: a Ser/Thr of the GRK family. Phosphorylates and inactivates photon-activated rhodopsin. LOF mutations lead to type 2 Oguchi disease, a form of night blindness. A link between RHOK and retinitis pigmentosa is now thought to be unlikely

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Protein kinase, AGC; EC 2.7.11.14; AGC group; GRK family; GRK subfamily

Chromosomal Location of Human Ortholog: 13q34

Molecular Function: G-protein coupled receptor kinase activity; rhodopsin kinase activity; ATP binding; protein kinase activity

Biological Process: rhodopsin mediated signaling; post-embryonic retina morphogenesis in camera-type eye; phototransduction, visible light; regulation of rhodopsin mediated signaling; visual perception; protein amino acid autophosphorylation; photoreceptor cell morphogenesis; regulation of G-protein coupled receptor protein signaling pathway; positive regulation of phosphorylation; negative regulation of apoptosis

Disease: Oguchi Disease 2