Rabbit anti-Human Hepcidin, pro Polyclonal Antibody | anti-HAMP antibody

Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide)

Cat. Num. AAA610429

• Gene Names: HAMP; HEPC; PLTR; HFE2B; LEAP1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Hepcidin; pro; Polyclonal Antibody; pro (Pro Hepc; LEAP; Hepatic Bactericidal Protein; Liver Expressed Antimicrobial Peptide); Anti -Hepcidin; anti-HAMP antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Hepcidin, pro (Pro Hepc, LEAP, Hepatic Bactericidal Protein, Liver Expressed Antimicrobial Peptide). Does not react with the secreted forms of HEPC. Species sequence homology: mouse and rat 63%.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-HAMP antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:100,000 using 50-100ng control peptide; H2009-02B per well.; Western Blot: 1-10ug/ml using Chemiluminescence.
• Immunogen: Synthetic peptide, 11-aa, corresponding to the N-terminus of human Pro-HEPC (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HAMP antibody

Hepcidin (Hepc, hepatic bactericidal protein) or LEAP (liver expressed antimicrobial peptide) is small, cysteine-rich peptide, antimicrobial peptide similar to defensins and thionins. Hepcidin (unprocessed, proprotein in mouse 83 aa, rat/human 84 aa) are almost exclusively produced in liver. Human hepcidin is produced from 84-aa precursor, including a putative 24-aa signal peptide. The signal peptide is cleaved to produce pro-HEPC, which is further processed to generate the mature secreted form of hepcidin (C-terminal 20, 22 or 25-aa HEPC). In humans, 20-aa and 25-aa appears to be the major HEPC secreted peptides with antimicrobial activities. The three secreted HEPC alternatively spliced HEPC peptides differ at the N-terminus.

Product Categories/Family for anti-HAMP antibody

Antibodies; Abs to Peptides

NCBI and Uniprot Product Information

• NCBI GI #: 10720397
• NCBI GeneID: 57817
• UniProt Accession #: P81172; Q1HE14; Q9BY68
• Molecular Weight: 9,408 Da
• NCBI Official Full Name: Hepcidin
• NCBI Official Synonym Full Names: hepcidin antimicrobial peptide
• NCBI Official Symbol: HAMP
• NCBI Official Synonym Symbols: HEPC; PLTR; HFE2B; LEAP1
• NCBI Protein Information: hepcidin; putative liver tumor regressor; liver-expressed antimicrobial peptide 1
• UniProt Protein Name: Hepcidin
• Protein Family: Hepcidin
• UniProt Gene Name: HAMP
• UniProt Synonym Gene Names: HEPC; LEAP1
• UniProt Entry Name: HEPC_HUMAN

NCBI Description

The product encoded by this gene is involved in the maintenance of iron homeostasis, and it is necessary for the regulation of iron storage in macrophages, and for intestinal iron absorption. The preproprotein is post-translationally cleaved into mature peptides of 20, 22 and 25 amino acids, and these active peptides are rich in cysteines, which form intramolecular bonds that stabilize their beta-sheet structures. These peptides exhibit antimicrobial activity. Mutations in this gene cause hemochromatosis type 2B, also known as juvenile hemochromatosis, a disease caused by severe iron overload that results in cardiomyopathy, cirrhosis, and endocrine failure. [provided by RefSeq]

Uniprot Description

HAMP: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. Defects in HAMP are the cause of hemochromatosis type 2B (HFE2B); also known as juvenile hemochromatosis (JH). HFE2B is a disorder of iron metabolism with excess deposition of iron in the tissues, bronze skin pigmentation, hepatic cirrhosis, arthropathy and diabetes. The most common symptoms of hemochromatosis type 2 at presentation are hypogonadism and cardiomyopathy. Belongs to the hepcidin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 19q13.1

Cellular Component: apical cortex; extracellular region

Molecular Function: hormone activity

Biological Process: cellular iron ion homeostasis; killing of cells of another organism; defense response to bacterium; immune response; defense response to fungus

Disease: Hemochromatosis, Type 2b