Rabbit anti-Canine Hakai Polyclonal Antibody | anti-Hakai antibody
Hakai
Gene Names
Hakai; CG10263; DmelCG10263; hakai
Reactivity
Canine
Applications
ELISA, Western Blot
Purity
PurifiedPurified by epitope affinity chromatography.
Synonyms
Hakai; Polyclonal Antibody; Anti -Hakai; anti-Hakai antibody
Host
Rabbit
Reactivity
Canine
Clonality
Polyclonal
Specificity
Recognizes canine Hakai. On Western blots, it identifies a single band at ~70kD, as well as a band at ~75-80kD that likely represents the ubiquitinated form of the Hakai protein. Reactivity has been confirmed with MDCK cell lysates. Species sequence homology: Mouse and human.
Purity/Purification
Purified
Purified by epitope affinity chromatography.
Purified by epitope affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Applicable Applications for anti-Hakai antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Dilution: ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml
Immunogen
Synthetic peptide derived from the C-terminal region of the human Hakai protein.
Positive Control
MDCK cell lysates
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Hakai antibody
Hakai was recently characterized as an E3 ubiquitin ligase related to the molecule c-Cbl1. c-Cbl is responsible for ubiquitinating the epithelial growth factor receptor (EGFR) and pro cadherins. Hakai is a novel E-cadherin binding protein, an E3 ligase which mediates ubiquitination of the E-cadherin complex in a tyrosine phosphorylation dependent manner. Hakai interacts with E-cadherin, but not with other cadherins such as N-cadherin or OB-cadherin. It has been reported to contain SH2, RING, zinc-finger and proline rich domains. There are indications that Hakai functions in the generation of cell motility under physiological conditions: first, over
Product Categories/Family for anti-Hakai antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
32,559 Da
NCBI Official Full Name
hakai, isoform F
NCBI Official Symbol
Hakai
NCBI Official Synonym Symbols
CG10263; DmelCG10263; hakai
NCBI Protein Information
CG10263 gene product from transcript CG10263-RE; CG10263-PA; CG10263-PC; CG10263-PE; CG10263-PF; Hakai-PA; Hakai-PC; Hakai-PE; Hakai-PF; hakai
UniProt Protein Name
Hakai, isoform A
UniProt Gene Name
Hakai
UniProt Entry Name
Q9VIT1_DROME
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Product Notes
The Hakai hakai (Catalog #AAA610267) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Hakai reacts with Canine and may cross-react with other species as described in the data sheet. AAA Biotech's Hakai can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 0.1-1ug/ml Western Blot: 1-3ug/ml. Researchers should empirically determine the suitability of the Hakai hakai for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Hakai, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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