Rabbit SLC1A5 Polyclonal Antibody | anti-SLC1A5 antibody

SLC1A5 Antibody

Cat. Num. AAA9612402

• Gene Names: SLC1A5; R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: SLC1A5; Polyclonal Antibody; SLC1A5 Antibody; ASCT2; AAAT; AAAT_HUMAN; ATB(0); ATBO; Baboon M7 virus receptor; FLJ31068; M7V1; M7VS1; Neutral amino acid transporter B(0); R16; RD114/simian type D retrovirus receptor; RDR; RDRC; Sodium dependent neutral amino acid transporter type 2; Sodium-dependent neutral amino acid transporter type 2; Solute carrier family 1 (neutral amino acid transporter); member 5; Solute carrier family 1 member 5; anti-SLC1A5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: SLC1A5 Antibody detects endogenous levels of total SLC1A5.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 541

• Applicable Applications for anti-SLC1A5 antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human SLC1A5, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from various samples, using SLC1A5 Antibody.Lane 1: Mouse testis, treated with blocking peptide;Lane 2: Mouse testis;Lane 3: Hybridoma cells.Observed bands: 45 kDa.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612402 at 1/100 staining Human esophageal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-SLC1A5 antibody

Function: Sodium-dependent amino acids transporter that has a broad substrate specificity, with a preference for zwitterionic amino acids. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated, anionic, and cationic amino acids (PubMed:8702519, PubMed:29872227). Through binding of the fusogenic protein syncytin-1/ERVW-1 may mediate trophoblasts syncytialization, the spontaneous fusion of their plasma membranes, an essential process in placental development (PubMed:10708449, PubMed:23492904).(Microbial infection) Acts as a cell surface receptor for Feline endogenous virus RD114.(Microbial infection) Acts as a cell surface receptor for Baboon M7 endogenous virus.(Microbial infection) Acts as a cell surface receptor for type D simian retroviruses.

Subcellular Location: Cell membrane>Multi-pass membrane protein. Melanosome. Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Tissue Specificity: Placenta, lung, skeletal muscle, kidney, pancreas, and intestine.

Subunit Structure: Homotrimer (Probable) (PubMed:29872227). Interacts with ERVH48-1/suppressyn; may negatively regulate syncytialization (PubMed:23492904).

Similarity: Belongs to the dicarboxylate/amino acid:cation symporter (DAACS) (TC 2.A.23) family. SLC1A5 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 5032093
• NCBI GeneID: 6510
• NCBI Accession #: NP_005619.1
• NCBI GenBank Nucleotide #: NP_005619.1
• UniProt Accession #: Q15758
• Molecular Weight: Observed Molecular Weight: (Observed)56kD, 45kD.; Predicted Molecular Weight: (Calculated)57kDa.
• NCBI Official Full Name: neutral amino acid transporter B(0) isoform 1
• NCBI Official Synonym Full Names: solute carrier family 1 member 5
• NCBI Official Symbol: SLC1A5
• NCBI Official Synonym Symbols: R16; AAAT; ATBO; M7V1; RDRC; ASCT2; M7VS1
• NCBI Protein Information: neutral amino acid transporter B(0)
• UniProt Protein Name: Neutral amino acid transporter B(0)
• Protein Family: Neutral amino acid transporter
• UniProt Gene Name: SLC1A5
• UniProt Synonym Gene Names: ASCT2; M7V1; RDR; RDRC; ATB(0)
• UniProt Entry Name: AAAT_HUMAN

NCBI Description

The SLC1A5 gene encodes a sodium-dependent neutral amino acid transporter that can act as a receptor for RD114/type D retrovirus (Larriba et al., 2001 [PubMed 11781704]).[supplied by OMIM, Jan 2011]

Uniprot Description

SLC1A5: Has a broad substrate specificity, a preference for zwitterionic amino acids, and a sodium-dependence. It accepts as substrates all neutral amino acids, including glutamine, asparagine, and branched-chain and aromatic amino acids, and excludes methylated amino acids, anionic amino acids, and cationic amino acids. Act as a cell surface receptor for feline endogenous virus RD114, baboon M7 endogenous virus and type D simian retroviruses. Placenta, lung, skeletal muscle, kidney, pancreas, and intestine. Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family. SLC1A5 subfamily. 1 isoforms of the human protein are produced by alternative initiation.

Protein type: Membrane protein, integral; Transporter; Transporter, SLC family; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: Golgi apparatus; membrane; integral to plasma membrane; melanosome; plasma membrane

Molecular Function: viral receptor activity; L-serine transmembrane transporter activity; protein binding; neutral amino acid transmembrane transporter activity; receptor activity; L-glutamine transmembrane transporter activity; sodium:dicarboxylate symporter activity

Biological Process: neutral amino acid transport; extracellular amino acid transport; entry of virus into host cell; amino acid transport; dicarboxylic acid transport; ion transport; glutamine transport; transmembrane transport; L-serine transport