Mouse anti-Human CD89 Monoclonal Antibody
CD89 (CD89 Antigen, FCAR, Immunoglobulin alpha Fc Receptor, IgA Fc Receptor)
Reactivity
Human
Applications
Flow Cytometry, Functional Assay, Immunofluorescence
Purity
PurifiedPurified.
Synonyms
CD89; Monoclonal Antibody; CD89 (CD89 Antigen; FCAR; Immunoglobulin alpha Fc Receptor; IgA Fc Receptor); Anti -CD89 (CD89 Antigen; anti-CD89 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
10K141
Specificity
Recognizes the EC1 domain of the human FcaRI extracellular region.
Purity/Purification
Purified
Purified.
Purified.
Form/Format
Supplied as a liquid in PBS, 0.1% sodium azide.
Applicable Applications for anti-CD89 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Flow Cytometry and Immunofluorescence.
Immunogen
Soluble CD89.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-CD89 antibody
FCAR, also called FcaRI or CD89, is a variably glycosylated 50-100kD myeloid-specific type I transmembrane (TM) Fc receptor for IgA that is a member of the multichain immune recognition receptor (MIRR) family. Human FCAR contains a 21aa signal sequence and extracellular (ECD), TM and cytoplasmic domains of 206, 19 and 41aa, respectively. Arg230 within the TM domain supports interaction with the ITAM-containing signaling subunit, FcRg, which contains a TM Asp. Two ECD C2-type Ig-like domains (EC1 and 2) are oriented at right angles. Up to two molecules of FCAR can bind one molecule of serum IgA via EC1. Many splice variants have been reported, but only two have been identified as proteins. The a.2 form, which lacks 22aa just prior to the TM domain, is exclusively expressed in alveolar macrophages. The a.3 form lacks EC2. FCAR binds monomeric, polymeric and secretory IgA, but does not mediate the barrier function of secretory IgA in mucosal epithelium. Shedding and circulation of polymeric IgA/FCAR immune complexes has been reported. Circulating neutrophils, eosinophils, and monocytes express FCAR. Tissue expression of FCAR is mainly from neutrophils; FCAR is downregulated as monocytes differentiate to tissue macrophages. On neutrophils, a significant amount of FCAR lacks FcRg, but can still be endocytosed to early endosomes and recycled to the cell surface. Binding of serum IgA to FCAR is transient and anti- inflammatory, inhibiting IgG or IgE-induced degranulation. Sustained aggregation of FCAR results in inflammatory responses. FcRg signaling is required for these and for transport to late endosomes. Human FCAR shows 55-58% aa identity with rat, horse and cow FCAR. No ortholog occurs in mouse. FCAR structure resembles the KIR/ILT/LIR/MIR family more than other IgA receptors, including pIgR, Fca/uR, asialoglycoprotein receptor (ASGR1) and transferrin receptor (TfR).
Product Categories/Family for anti-CD89 antibody
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Product Notes
The CD89 (Catalog #AAA609433) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD89 (CD89 Antigen, FCAR, Immunoglobulin alpha Fc Receptor, IgA Fc Receptor) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD89 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Flow Cytometry and Immunofluorescence. Researchers should empirically determine the suitability of the CD89 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD89, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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