Mouse TAF12 Monoclonal Antibody | anti-TAF12 antibody

Anti-TAF12 Antibody

Cat. Num. AAA415242

• Gene Names: TAF12; TAF2J; TAFII20
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: TAF12; Monoclonal Antibody; Anti-TAF12 Antibody; anti-TAF12 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Specificity: Anti- TAF12 recognizes TAF12 expression in Hela whole cell lysate.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 1129

• Applicable Applications for anti-TAF12 antibody: ELISA (EIA), Western Blot (WB-Ce)
• Application Notes: WB: 1:500, analysis of TAF12 expression in Hela whole cell lysate with Anti- TAF12
• Raised In: Purified mouse monoclonal antibody generated using E.coli expressed recombinant protein TAF12 as immunogen.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Immunogen: Recombinant protein corresponding to full length TAF12.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

None

NCBI and Uniprot Product Information

• NCBI GI #: 206725448
• NCBI GeneID: 6883
• NCBI Accession #: NP_005635.1
• NCBI GenBank Nucleotide #: NM_005644
• Molecular Weight: 14,825 Da
• NCBI Official Full Name: Homo sapiens TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa (TAF12), transcript variant 2, mRNA
• NCBI Official Synonym Full Names: TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa
• NCBI Official Symbol: TAF12
• NCBI Official Synonym Symbols: TAF2J; TAFII20
• NCBI Protein Information: transcription initiation factor TFIID subunit 12; TAFII-20/TAFII-15; TAFII20/TAFII15; TATA box binding protein (TBP)-associated factor, RNA polymerase II, J, 20kD; transcription initiation factor TFIID 20/15 kDa subunits
• UniProt Protein Name: Transcription initiation factor TFIID subunit 12
• Protein Family: Transcription initiation factor
• UniProt Gene Name: TAF12
• UniProt Entry Name: TAF12_HUMAN

NCBI Description

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

TAF12: TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. Belongs to the TAF12 family. 2 isoforms of the human protein are produced by alternative initiation.

Protein type: Transcription initiation complex

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: nucleoplasm; PCAF complex; transcription factor TFIID complex

Molecular Function: protein binding; histone acetyltransferase activity; DNA binding; protein heterodimerization activity; transcription coactivator activity; transcription factor activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; viral reproduction; transcription initiation; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription factor activity