Testing Data
(Live confocal imaging of intracellular trafficking of p75NTR using ATTO 488 labelled mouse monoclonal antibody to human p75NTR in NSC34 mouse motor neuron cell line.)
Mouse Nerve Growth Factor Receptor, p75 Monoclonal Antibody | anti-NGFR antibody
Nerve Growth Factor Receptor, p75 (NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16) (ATTO 488)
Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
Purity
Affinity PurifiedPurified by affinity chromatography.
Synonyms
Nerve Growth Factor Receptor; p75; Monoclonal Antibody; p75 (NGFR; NGF Receptor; CD271; Gp80 LNGFR; p75 ICD; p75 Neurotrophin; p75 NTR; Tumor Necrosis Factor Receptor Superfamily Member 16; TNFR Superfamily Member 16; TNFRSF16) (ATTO 488); Anti -Nerve Growth Factor Receptor; anti-NGFR antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG2A
Clone Number
9G393
Specificity
Recognizes human p75NTR (low affinity neurotrophin receptor). Species Crossreactivity: mouse and rat. It is expected that this antibody will be useful for the study of p75 in primates and other species.
Purity/Purification
Affinity Purified
Purified by affinity chromatography.
Purified by affinity chromatography.
Form/Format
Supplied as a liquid. Conjugated to ATTO 488.
Applicable Applications for anti-NGFR antibody
ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunohistochemistry, Immunofluorescence, Flow Cytometry, Anti-NGF Receptor p75 Dynamics, Retrograde Transport Studies, and Study of Intracellular Trafficking. Not suitable for use in Western Blot or for blocking Neurotrophin binding.
Dilution: Immunohistochemistry: 1-2ug/ml
Flow Cytometry: 20ug/ml
ELISA: 1:5000
Optimal dilution determined by the researcher.
Dilution: Immunohistochemistry: 1-2ug/ml
Flow Cytometry: 20ug/ml
ELISA: 1:5000
Optimal dilution determined by the researcher.
Immunogen
Human recombinant p75.
Preparation and Storage
May be stored at 4 degree C. For long-term storage, aliquot and store at 4 degree C. Do not freeze. Aliquots are stable for up to 4 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Note: We do not recommended adding glycerol as a cryopreservative.
Note: We do not recommended adding glycerol as a cryopreservative.
Testing Data
(Live confocal imaging of intracellular trafficking of p75NTR using ATTO 488 labelled mouse monoclonal antibody to human p75NTR in NSC34 mouse motor neuron cell line.)
Testing Data
(Live confocal imaging of intracellular trafficking of p75NTR using ATTO 488 labelled mouse monoclonal antibody to human p75NTR in NSC34 mouse motor neuron cell line.)
Testing Data
(Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.)
Testing Data
(Photostability of ATTO 488 compared to common dyes in water. Excitation at 488nm with 1W Argon-Ion Laser.)
Related Product Information for anti-NGFR antibody
p75NTR was originally discovered as a low affinity nerve growth factor receptor. Later it was found that it was the receptor for all neurotrophins. It mediates signals of neurotrophins for neuronal survival, apoptosis, neurite outgrowth and synaptic plasticity. Recently, it has been revealed that p75NTR is not only acts as the receptor for neurotrophins but also the receptor for many other pathological ligands such as prions, rabies virus and amyloid beta. p75NTR also acts as a co-receptor for NOGO which mediates inhibitory signals of myelin associated protein. p75NTR is highly expressed in a number of non-neuronal and neuronal cells including motor neurons during development and also in damaged neurons. FUNCTION: Low affinity receptor which can bind to NGF, BDNF, NT-3, and NT-4. Can mediate cell survival as well as cell death of neural cells. SUBUNIT: Homodimer; disulfide-linked. Interacts with p75NTR-associated cell death executor.
Product Categories/Family for anti-NGFR antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
nerve growth factor receptor
NCBI Official Symbol
NGFR
NCBI Protein Information
nerve growth factor receptor
Protein Family
Similar Products
Product Notes
The NGFR (Catalog #AAA608174) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Nerve Growth Factor Receptor, p75 (NGFR, NGF Receptor, CD271, Gp80 LNGFR, p75 ICD, p75 Neurotrophin, p75 NTR, Tumor Necrosis Factor Receptor Superfamily Member 16, TNFR Superfamily Member 16, TNFRSF16) (ATTO 488) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Nerve Growth Factor Receptor, p75 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF). Suitable for use in Immunohistochemistry, Immunofluorescence, Flow Cytometry, Anti-NGF Receptor p75 Dynamics, Retrograde Transport Studies, and Study of Intracellular Trafficking. Not suitable for use in Western Blot or for blocking Neurotrophin binding. Dilution: Immunohistochemistry: 1-2ug/ml Flow Cytometry: 20ug/ml ELISA: 1:5000 Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the NGFR for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nerve Growth Factor Receptor, p75, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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