Mouse anti-Human, Yeast RNA Polymerase IID Repeat Monoclonal Antibody | anti-POLR2E antibody

RNA Polymerase II, CTD Repeat (YSPTSPS) (RNA Pol II CTD, RNA Pol II-CTD, DNA-directed RNA Polymerase II Largest Subunit, RPB1, Pol2)

Cat. Num. AAA608118

• Gene Names: POLR2E; RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• Reactivity: Human, Yeast
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RNA Polymerase IID Repeat; Monoclonal Antibody; RNA Polymerase II; CTD Repeat (YSPTSPS) (RNA Pol II CTD; RNA Pol II-CTD; DNA-directed RNA Polymerase II Largest Subunit; RPB1; Pol2); Anti -RNA Polymerase II; anti-POLR2E antibody

• Host: Mouse
• Reactivity: Human, Yeast
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 9C82
• Specificity: This antibody recognizes the C-terminal repeat of the largest subunit of RNA polymerase II, it recognizes both unphosphorylated and phosphorylated forms. Species Crossreactivity: Crossreacts with Human, S. cerevisiae. Expected to crossreact with Drosophilia melanogaster, Hamster, Mouse, S. pombe, Arabidopsis thaliana due to sequence homology - all of these species contain at least one or more 100% identical repeats.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added.

• Applicable Applications for anti-POLR2E antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA Western Blot and Immunoprecipitation.
• Immunogen: 10 repeats of synthetic peptide YSPTSPS using chemically synthesized phospho-ser 5 YSPTSpPS (Human).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-POLR2E antibody

RNAPII is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII. CTD4H8 can also be used in chromatin immunoprecipitation (ChIP) assays.

Product Categories/Family for anti-POLR2E antibody

Antibodies; Abs to Enzymes; Polymerase

NCBI and Uniprot Product Information

• NCBI GI #: 232954
• NCBI GeneID: 5434
• UniProt Accession #: P19388; O43380; Q6PIH5; Q9BT06; B2R6L4; D6W5Y1
• Molecular Weight: 24,551 Da
• NCBI Official Full Name: RNA polymerase II
• NCBI Official Synonym Full Names: polymerase (RNA) II (DNA directed) polypeptide E, 25kDa
• NCBI Official Symbol: POLR2E
• NCBI Official Synonym Symbols: RPB5; XAP4; RPABC1; hRPB25; hsRPB5
• NCBI Protein Information: DNA-directed RNA polymerases I, II, and III subunit RPABC1; RPB5 homolog; DNA-directed RNA polymerase II subunit E; RNA polymerases I, II, and III subunit ABC1; DNA directed RNA polymerase II 23 kda polypeptide; DNA-directed RNA polymerase II 23 kDa polypeptide
• UniProt Protein Name: DNA-directed RNA polymerases I, II, and III subunit RPABC1
• UniProt Gene Name: POLR2E
• UniProt Entry Name: RPAB1_HUMAN

NCBI Description

This gene encodes the fifth largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit is shared by the other two DNA-directed RNA polymerases and is present in two-fold molar excess over the other polymerase subunits. An interaction between this subunit and a hepatitis virus transactivating protein has been demonstrated, suggesting that interaction between transcriptional activators and the polymerase can occur through this subunit. A pseudogene is located on chromosome 11. [provided by RefSeq]

Uniprot Description

POLR2E: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, POLR2E/RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process. Belongs to the archaeal RpoH/eukaryotic RPB5 RNA polymerase subunit family.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; DNA-directed RNA polymerase III complex; DNA-directed RNA polymerase II, core complex; nucleus; cytosol; DNA-directed RNA polymerase I complex

Molecular Function: protein binding; DNA binding; DNA-directed RNA polymerase activity

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; termination of RNA polymerase III transcription; viral reproduction; somatic stem cell maintenance; positive regulation of viral transcription; RNA splicing; transcription from RNA polymerase I promoter; RNA elongation from RNA polymerase I promoter; DNA repair; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transcription from RNA polymerase III promoter; nuclear mRNA splicing, via spliceosome; mRNA capping; nucleotide-excision repair; negative regulation of gene expression, epigenetic; transcription-coupled nucleotide-excision repair; RNA elongation from RNA polymerase II promoter; positive regulation of interferon type I production; innate immune response; gene expression; transcription initiation from RNA polymerase I promoter; RNA elongation from RNA polymerase III promoter