Rat anti-Mouse Interleukin 33 Propeptide Monoclonal Antibody | anti-Il33 antibody

Interleukin 33 Propeptide (IL-33, C9orf26, DKFZp586H0523, DVS27, IL1F11, IL-1F11, Interleukin-1 Family Member 11, Interleukin-33, NFEHEV, NFHEV, NF-HEV, Nuclear Factor from High Endothelial Venules, RP11-575C20.2)

Cat. Num. AAA607622

• Gene Names: Il33; Il-33; Il1f11; NF-HEV; 9230117N10Rik
• Reactivity: Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Interleukin 33 Propeptide; Monoclonal Antibody; Interleukin 33 Propeptide (IL-33; C9orf26; DKFZp586H0523; DVS27; IL1F11; IL-1F11; Interleukin-1 Family Member 11; Interleukin-33; NFEHEV; NFHEV; NF-HEV; Nuclear Factor from High Endothelial Venules; RP11-575C20.2); Anti -Interleukin 33 Propeptide (IL-33; anti-Il33 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 9H138
• Specificity: Recognizes mouse IL-33 Propeptide. Does not crossreact with mouse or human rIL-33, or the pro region of human rIL-33.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 0.2ml sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-Il33 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 0.5-1.0ug/ml; Detection limit for rmIL-33 is ~5ng/well.; Western Blot: 2ug/ml
• Immunogen: Recombinant mouse IL-33 Propeptide aa1-108; Q8BVZ5 (E. coli).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile 40-50% glycerol, PBS. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Il33 antibody

IL-33, also known as NF-HEV and DVS 27, is an IL-1 family member that is constitutively expressed in smooth muscle and airway epithelia and is upregulated by IL-1 alpha or beta in arterial smooth muscle, dermal fibroblasts and keratinocytes. IL-33 is synthesized as a 266aa, 32kD proform that localizes to the nucleus and may regulate gene transcription. Cleavage generates mature IL-33 as an 18kD C-terminal proinflammatory fragment which binds IL-1 R4/ST2 on Th2 and mast cells. Mature mouse IL-33 shares 55% and 90% amino acid sequence identity with human and rat IL-33, respectively.

Product Categories/Family for anti-Il33 antibody

Antibodies; Abs to Interleukin

NCBI and Uniprot Product Information

• NCBI GI #: 68565902
• NCBI GeneID: 77125
• UniProt Accession #: Q8BVZ5; Q2YEJ4; Q3TQN0; Q99L46
• Molecular Weight: 29,991 Da
• NCBI Official Full Name: Interleukin-33
• NCBI Official Synonym Full Names: interleukin 33
• NCBI Official Symbol: Il33
• NCBI Official Synonym Symbols: Il-33; Il1f11; NF-HEV; 9230117N10Rik
• NCBI Protein Information: interleukin-33; OTTMUSP00000035769; nuclear factor from high endothelial venules
• UniProt Protein Name: Interleukin-33
• Protein Family: Interleukin
• UniProt Gene Name: Il33
• UniProt Entry Name: IL33_MOUSE

Uniprot Description

IL33: Cytokine that binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Induces T-helper type 2-associated cytokines. Belongs to the IL-1 family. Highly divergent. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytokine

Cellular Component: extracellular space; extracellular region; cytoplasmic vesicle; chromosome; nucleus

Molecular Function: cytokine activity

Biological Process: negative regulation of T-helper 1 type immune response; positive regulation of T-helper 2 type immune response; transcription, DNA-dependent; positive regulation of interleukin-6 production; positive regulation of immunoglobulin secretion; negative regulation of leukocyte migration; positive regulation of interleukin-4 production; negative regulation of immunoglobulin secretion; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; positive regulation of interleukin-13 production; defense response to virus; positive regulation of interleukin-5 production; positive regulation of macrophage activation; positive regulation of inflammatory response